Wang E Y, Giclas P C, Tu R H, Hata C, Quijano R C
Baxter Healthcare Corporation, Irvine, California.
ASAIO J. 1993 Jul-Sep;39(3):M691-4.
This study was performed to evaluate the degree of complement activation by three bovine arterial graft materials: Bioflow (Bio-Vascular Inc., a bovine artery fixed with dialdehyde starch), BioPolyMeric (St. Jude Medical Inc., a collagen conduit of bovine arterial origin, tanned with glutaraldehyde and covered with a Dacron mesh), and Denaflex (Baxter Edwards CVS Division, a bovine artery fixed with polyepoxy compounds). The grafts were rinsed by following the manufacturer's recommended procedures and thereafter incubated with normal human serum. CH50 assays were performed on the serum after incubation, and the percentage of complement activation for each sample was calculated relative to its control serum. The results indicated that the BioPolyMeric grafts activated the most complement, with about a 48% decrease in the CH50. The BioPolyMeric graft is composed of an outer polyester mesh and an inner collagenous tubing, exhibiting a nonreversible negative surface charge. After the polyester mesh was removed, the BioPolyMeric graft showed the highest complement activation in this study, suggesting that the glutaraldehyde fixed graft is more prone to complement activation than either the polyepoxy compound or dialdehyde starch fixed grafts. The complement fragment, C5a, generated during complement activation is strongly chemotactic for polymorphonuclear leukocytes and monocytes, which likely play early and long-lasting roles in regulating tissue reaction to the implanted graft.
Bioflow（Bio-Vascular公司，用二醛淀粉固定的牛动脉）、BioPolyMeric（圣犹达医疗公司，牛动脉来源的胶原导管，用戊二醛鞣制并覆盖涤纶网）和Denaflex（百特爱德华兹心血管事业部，用多环氧化合物固定的牛动脉）。按照制造商推荐的程序冲洗移植物，然后与正常人血清孵育。孵育后对血清进行CH50测定，并计算每个样品相对于其对照血清的补体激活百分比。结果表明，BioPolyMeric移植物激活的补体最多，CH50降低约48%。BioPolyMeric移植物由外层聚酯网和内层胶原管组成，呈现不可逆的负表面电荷。去除聚酯网后，BioPolyMeric移植物在本研究中显示出最高的补体激活，这表明戊二醛固定的移植物比多环氧化合物或二醛淀粉固定的移植物更容易激活补体。补体激活过程中产生的补体片段C5a对多形核白细胞和单核细胞具有强烈的趋化作用，它们可能在调节组织对植入移植物的反应中发挥早期和持久的作用。
