Scott M J, Kriticou D, Robinson A S
Insect Molecular Genetics Group, Institute of Molecular Biology and Biotechnology, Heraklion, Crete, Greece.
Insect Mol Biol. 1993;1(4):213-22. doi: 10.1111/j.1365-2583.1993.tb00094.x.
We have isolated and determined the nucleotide sequences for cDNA clones encoding glucose-6-phosphate dehydrogenase (G6PD) and 6-phosphogluconate dehydrogenase (6PGD) from the medfly Ceratitis capitata. The derived amino acid sequences for G6PD and 6PGD are presented and compared with G6PDs and 6PGDs from other species. The codon usage of the cDNA clones has little bias with the notable exceptions of arginine, glycine and leucine. The chromosomal location of the genes for 6PGD and G6PD were determined by in situ hybridization to salivary gland polytene chromosomes. This localization orients a genetic map of enzymatic loci and illustrates a remarkable similarity in the intra chromosomal order of homologous genes between Drosophila melanogaster and medfly.
我们已经从地中海实蝇（Ceratitis capitata）中分离并确定了编码葡萄糖-6-磷酸脱氢酶（G6PD）和6-磷酸葡萄糖酸脱氢酶（6PGD）的cDNA克隆的核苷酸序列。给出了推导的G6PD和6PGD氨基酸序列，并与其他物种的G6PD和6PGD进行了比较。cDNA克隆的密码子使用偏好性很小，只有精氨酸、甘氨酸和亮氨酸是明显的例外。通过对唾液腺多线染色体进行原位杂交，确定了6PGD和G6PD基因的染色体定位。这种定位确定了酶基因座的遗传图谱，并说明了黑腹果蝇和地中海实蝇之间同源基因在染色体内顺序上的显著相似性。
