Boedigheimer M, Laughon A
Laboratory of Genetics, University of Wisconsin, Madison 53706.
Development. 1993 Aug;118(4):1291-301. doi: 10.1242/dev.118.4.1291.
The expanded gene was first identified by a spontaneous mutation that causes broad wings. We have identified an enhancer-trap insertion within expanded and used it to generate additional mutations, including one null allele. expanded is an essential gene, necessary for proper growth control of imaginal discs and, when mutant, causes either hyperplasia or degeneration depending on the disc. Wing overgrowth in expanded hypermorphs is limited to specific regions along the anterior-posterior and dorsal-ventral axis. expanded encodes a novel 1429 amino acid protein that is localized to the apical surface of disc cells and contains three potential SH3-binding sites. Together, these observations suggest that the Expanded protein engages in protein-protein interactions regulating cell proliferation in discs.
最初通过导致翅膀变宽的自发突变鉴定出了这个扩展基因。我们在扩展基因内鉴定到一个增强子陷阱插入，并利用它产生了其他突变，包括一个无效等位基因。扩展基因是一个必需基因，对成虫盘的正常生长控制至关重要，当发生突变时，根据成虫盘的不同会导致增生或退化。扩展基因超形态中的翅膀过度生长仅限于前后轴和背腹轴的特定区域。扩展基因编码一种新的1429个氨基酸的蛋白质，该蛋白质定位于成虫盘细胞的顶端表面，并含有三个潜在的SH3结合位点。这些观察结果共同表明，扩展蛋白参与调节成虫盘细胞增殖的蛋白质-蛋白质相互作用。
