Di Martino A, Safar J, Ceroni M, Gibbs C J
Fidia Research Laboratories, Abano Terme, Italy.
Dev Biol Stand. 1993;80:187-94.
The inactivation of the scrapie agent in biologicals was evaluated on a scaled-down procedure currently in use on a large scale for the purification of gangliosides from bovine brain tissue. Residual infectivity was determined by an in vivo animal bio-assay using hamsters as the host species and the 263K strain as the reference agent. Infectivity was significantly diminished during the early stages of purification and infectivity was never detected in the final preparations. These results confirmed that a combination of chemicals or physical techniques known to affect the viability of the scrapie agent led to complete inactivation.
在目前大规模用于从牛脑组织中纯化神经节苷脂的缩微程序上，对生物制品中瘙痒病病原体的灭活情况进行了评估。使用仓鼠作为宿主物种，以263K毒株作为参考病原体，通过体内动物生物测定法来确定残余感染力。在纯化的早期阶段，感染力显著降低，并且在最终制品中从未检测到感染力。这些结果证实，已知会影响瘙痒病病原体活力的化学物质或物理技术的组合可导致完全灭活。
