Resnick S M, Torok D S, Gibson D T
Department of Microbiology, College of Medicine, University of Iowa, Iowa City 52242.
FEMS Microbiol Lett. 1993 Nov 1;113(3):297-302. doi: 10.1111/j.1574-6968.1993.tb06530.x.
Naphthalene 1,2-dioxygenase from Pseudomonas sp. NCIB 9816-4 and biphenyl dioxygenase from Beijerinckia sp. B8/36 oxidized the aromatic N-heterocycle carbazole to 3-hydroxycarbazole. Toluene dioxygenase from Pseudomonas putida F39/D did not oxidize carbazole. Transformations were carried out by mutant strains which oxidize naphthalene and biphenyl to cis-dihydrodiols, and with a recombinant E. coli strain expressing the structural genes of naphthalene 1,2-dioxgenase from Pseudomonas sp. NCIB 9816-4. 3-Hydroxycarbazole is presumed to result from the dehydration of an unstable cis-dihydrodiol.
来自假单胞菌属NCIB 9816 - 4的萘1,2 - 双加氧酶和来自拜叶林克氏菌属B8/36的联苯双加氧酶将芳香族氮杂环咔唑氧化为3 - 羟基咔唑。来自恶臭假单胞菌F39/D的甲苯双加氧酶不能氧化咔唑。转化是由将萘和联苯氧化为顺式二氢二醇的突变菌株，以及表达来自假单胞菌属NCIB 9816 - 4的萘1,2 - 双加氧酶结构基因的重组大肠杆菌菌株进行的。推测3 - 羟基咔唑是由不稳定的顺式二氢二醇脱水产生的。
