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鸡（原鸡）中耳上皮的精细结构

Fine structure of the middle ear epithelium in the chicken (Gallus gallus).

作者信息

Giannessi F, Ruffoli R

机构信息

Istituto di Anatomia Normale, Università degli Studi di Pisa, Italy.

出版信息

J Anat. 1993 Aug;183 ( Pt 1)(Pt 1):103-11.

PMID:8270465
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1259858/
Abstract

The epithelium lining the tympanic cavity of the chicken possesses distinct morphological characteristics. Its ultrastructure was studied using 2 preparative techniques. (1) After fixation in Karnovsky's solution, postfixation in osmium tetroxide and embedding in Epon, the epithelium was observed to contain 2 kinds of cell: secretory and basal. The secretory cells (which we refer to as mixed granulated cells) showed numerous secretory vesicles that varied in appearance, some containing paracrystalline formations. The basal cells, located close to the basement membrane, showed no evidence of secretory activity. (2) Other specimens were immersed in Karnovsky fixative and subsequently in a mixture of glutaraldehyde and tannic acid. They were then osmicated and embedded in polar Epon mix. With this method, the epithelium was seen to be covered by electron-dense material made up of thin intertwined tubules. In addition, the secretory cells contained vesicles with concentrically arranged lamellae; such vesicles resembled the multilamellar bodies of mammalian type II pneumocytes. The hypothesis is advanced that tubules and lamellar vesicles are related to the presence of surfactant substances.

摘要

鸡鼓室的上皮具有明显的形态学特征。利用两种制备技术对其超微结构进行了研究。（1）用卡诺夫斯基溶液固定、四氧化锇后固定并包埋于环氧树脂中后，观察到上皮含有两种细胞：分泌细胞和基底细胞。分泌细胞（我们称之为混合颗粒细胞）显示出许多外观各异的分泌小泡，有些含有平行排列的晶体结构。位于靠近基底膜处的基底细胞未显示出分泌活性的迹象。（2）其他标本先浸入卡诺夫斯基固定液，随后浸入戊二醛和鞣酸的混合液中。然后进行锇酸处理并包埋于极性环氧树脂混合物中。用这种方法，可以看到上皮被由细的交织小管组成的电子致密物质所覆盖。此外，分泌细胞含有具有同心排列板层的小泡；这种小泡类似于哺乳动物II型肺细胞的多层小体。有人提出假说，认为小管和板层小泡与表面活性物质的存在有关。

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A morphologic and cytochemical study on the great alveolar cell.关于肺泡大细胞的形态学和细胞化学研究。
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