两步聚合酶链反应检测嗜肺军团菌的研究

[Study of Legionella pneumophila detection by two step polymerase chain reaction].

作者信息

Koide M, Saito A, Higa F, Yamashiro Y, Ishimine T, Futenma M, Inadome J, Kawakami K, Kusano N

机构信息

First Department of Internal Medicine, Faculty of Medicine, University of the Ryukyus.

出版信息

Kansenshogaku Zasshi. 1993 Nov;67(11):1062-7. doi: 10.11150/kansenshogakuzasshi1970.67.1062.

DOI:10.11150/kansenshogakuzasshi1970.67.1062

PMID:8270798

Abstract

We studied the usefulness of two sets of primers in macrophage infectivity potentiator gene sequence of Legionella pneumophila. PCR by 1st step primers produced 649 bp DNA bands and 2nd step primers 489 bp DNA bands. Two step PCR by these primers produced 489 bp DNA bands specific for L. pneumophila. Two step PCR detected 10 fg of pure DNA extracted from L. pneumophila, Philadelphia-1 strain. PCR sensitivity by these primers was superior to former primers reported by us using intratracheal aspirates collected from the patient with L. pneumophila serogroup 2 pneumonia.

摘要

我们研究了两组引物在嗜肺军团菌巨噬细胞感染增强子基因序列中的效用。第一步引物进行的PCR产生了649 bp的DNA条带，第二步引物产生了489 bp的DNA条带。由这些引物进行的两步PCR产生了嗜肺军团菌特异的489 bp DNA条带。两步PCR检测到了从嗜肺军团菌费城-1菌株提取的10 fg纯DNA。这些引物的PCR敏感性优于我们之前报道的使用从嗜肺军团菌血清型2肺炎患者收集的气管吸出物的引物。