人成纤维细胞中的体积调节：Ca2+和5-脂氧合酶产物在氯离子外流激活中的作用。
Volume regulation in human fibroblasts: role of Ca2+ and 5-lipoxygenase products in the activation of the Cl- efflux.
作者信息
Mastrocola T, Lambert I H, Kramhøft B, Rugolo M, Hoffmann E K
机构信息
Laboratory of Biological Chemistry A, August Krogh Institute, Copenhagen, Denmark.
出版信息
J Membr Biol. 1993 Oct;136(1):55-62. doi: 10.1007/BF00241489.
Trypsinized human skin fibroblasts in suspension perform regulatory volume decrease (RVD) after cell swelling in hypotonic medium. During RVD, 36Cl- efflux is dramatically increased and the cell membrane is depolarized, indicating the activation of Cl- channels. This activation of Cl- channels depends on extracellular as well as on intracellular Ca2+. The swelling-induced Cl- efflux and the RVD response are inhibited by the 5-lipoxygenase inhibitor ETH 615-139. Finally, following hypotonic treatment, cellular pH decreases. The pH decrease does not involve the Cl-/HCO3- exchange because it is independent of the external Cl- concentration.
悬浮状态下经胰蛋白酶处理的人皮肤成纤维细胞在低渗培养基中发生细胞肿胀后会进行调节性容积减小（RVD）。在RVD过程中，³⁶Cl⁻外流显著增加，细胞膜发生去极化，表明氯离子通道被激活。这种氯离子通道的激活依赖于细胞外以及细胞内的钙离子。5-脂氧合酶抑制剂ETH 615-139可抑制肿胀诱导的氯离子外流和RVD反应。最后，在低渗处理后，细胞内pH值降低。pH值降低不涉及Cl⁻/HCO₃⁻交换，因为它与细胞外氯离子浓度无关。
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