Détári L, Kukorelli T, Hajnik T
Department of Comparative Physiology, Eötvös Loránd University, Budapest, Hungary.
J Neurosci Methods. 1993 Sep;49(3):225-30. doi: 10.1016/0165-0270(93)90127-d.
Several techniques were developed to prevent sleep in animals in order to examine the biological role fulfilled by sleep; however, most were either stressful or difficult to accomplish routinely, especially in such a large animal as the cat. Electrical stimulation of activating structures in the brain presents a very attractive alternative to peripheral stimulation used by the usual sleep deprivation methods although it has been rarely tried. The paper describes a microcomputer-based system used to achieve sleep deprivation in cats by stimulating the hypothalamic predatory area with short trains. During control days and deprivation the electrocorticogram (EEG), electromyogram (EMG) and electrooculogram (EOG) were continuously digitalized by the computer in 5 s epochs and the integrated power of the 4 usual frequency bands of the EEG (alpha, beta, delta, theta) as well as the variance of EMG and EOG signals were calculated. Criteria for stimulus delivery were based on the integrated power of the delta band and on the variance of EMG but the flexibility of the computer ensures that any other parameter can be used to achieve total or selective sleep deprivation.
为了研究睡眠所起的生物学作用，人们开发了多种防止动物睡眠的技术；然而，大多数技术要么压力大，要么难以常规操作，尤其是对于像猫这样的大型动物。与常用的睡眠剥夺方法所使用的外周刺激相比，对大脑激活结构进行电刺激是一种非常有吸引力的替代方法，尽管很少有人尝试过。本文描述了一种基于微型计算机的系统，该系统通过用短串刺激下丘脑捕食区来使猫睡眠剥夺。在对照日和剥夺期间，计算机以5秒的时段对脑电图（EEG）、肌电图（EMG）和眼电图（EOG）进行连续数字化处理，并计算EEG四个常用频段（α、β、δ、θ）的积分功率以及EMG和EOG信号的方差。刺激发放的标准基于δ频段的积分功率和EMG的方差，但计算机的灵活性确保可以使用任何其他参数来实现完全或选择性睡眠剥夺。
