经上皮二肽（甘氨酰肌氨酸）跨人Caco-2细胞单层上皮的转运是生电的。
Transepithelial dipeptide (glycylsarcosine) transport across epithelial monolayers of human Caco-2 cells is rheogenic.
作者信息
Thwaites D T, McEwan G T, Hirst B H, Simmons N L
机构信息
Department of Physiological Sciences, University of Newcastle upon Tyne, Medical School, UK.
出版信息
Pflugers Arch. 1993 Oct;425(1-2):178-80. doi: 10.1007/BF00374520.
Net transepithelial transport (and cellular accumulation) of the dipeptide glycylsarcosine (Gly-Sar), across the apical membrane of human intestinal Caco-2 epithelia, is driven by a proton gradient (Na(+)-free conditions) and displays saturation kinetics (Km 17.4 +/- 5.1 mM, Vmax of 92.8 +/- 15.6 nmol.cm-2.h-1). Net Gly-Sar transport is associated with the stimulation of an inward short-circuit current (Isc). This dipeptide-stimulated Isc is observed in both Na(+)-containing and Na(+)-free conditions, is stimulated by apical acidity, and displays saturation kinetics (in Na(+)-free media at apical pH 6.0, Km of 13.6 +/- 4.5 mM and a Vmax of 284.1 +/- 39.3 nmol.cm-2.h-1). The maximal capacities of Gly-Sar transport and Isc suggest a dipeptide/proton stoichiometry greater than unity (1:3).
二肽甘氨酰肌氨酸（Gly-Sar）在人肠道Caco-2上皮细胞顶膜上的净跨上皮转运（和细胞积累）由质子梯度驱动（无钠条件下），并呈现饱和动力学（Km为17.4±5.1 mM，Vmax为92.8±15.6 nmol·cm-2·h-1）。Gly-Sar的净转运与内向短路电流（Isc）的刺激有关。这种二肽刺激的Isc在含钠和无钠条件下均能观察到，受顶膜酸度刺激，并呈现饱和动力学（在无钠培养基中，顶膜pH 6.0时，Km为13.6±4.5 mM，Vmax为284.1±39.3 nmol·cm-2·h-1）。Gly-Sar转运和Isc的最大容量表明二肽/质子化学计量比大于1（1:3）。
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