Humphrey W R, Simmons C A, Toombs C F, Shebuski R J
Department of Cardiovascular Diseases Research, Upjohn Laboratories, Kalamazoo, MI 49001.
Am Heart J. 1994 Jan;127(1):20-31. doi: 10.1016/0002-8703(94)90505-3.
We evaluated the use of simple balloon overinflation to induce neointimal hyperplasia in a porcine model of coronary artery restenosis. By using standard percutaneous transluminal coronary angioplasty techniques, left anterior descending (LAD) and/or left circumflex (LCX) coronary arteries of either juvenile feeder pigs or adult Yucatan minipigs were intentionally overinflated. Four weeks later, resultant neointimal hyperplastic responses (neointima/media area; NI/M) were quantitated morphometrically. At all ballooned sites neointimal hyperplasia occurred only when the internal elastic lamina (IEL) was ruptured; the degree of hyperplasia correlated directly with the injury index, that is, the percentage of IEL circumference that fractured (r = 0.74; n = 25; p < 0.05). Despite similar injury indexes in the LAD bed, there was a trend (p = 0.07; analysis of variance) toward greater NI/M ratios in the Yucatan minipig versus the feeder pig group (1.14 +/- 0.21 vs 0.73 +/- 0.09, n = 7/group). We found no such trend in the LCX bed, where the injury index (25.7% +/- 3.5%) was significantly greater than that of the LAD (18.2% +/- 1.2%, p < 0.05). If variations in balloon-induced vascular injury are accounted for, the technique of balloon overinflation of coronary arteries should prove useful in testing potential antirestenotic agents in either adult or juvenile pigs.
我们评估了在猪冠状动脉再狭窄模型中使用简单球囊过度充盈诱导内膜增生的情况。通过使用标准的经皮腔内冠状动脉成形术技术，对幼年饲养猪或成年尤卡坦小型猪的左前降支（LAD）和/或左旋支（LCX）冠状动脉有意进行过度充盈。四周后，通过形态计量学对由此产生的内膜增生反应（内膜/中膜面积；NI/M）进行定量。在所有球囊扩张部位，仅当内弹力层（IEL）破裂时才会发生内膜增生；增生程度与损伤指数直接相关，即IEL周长破裂的百分比（r = 0.74；n = 25；p < 0.05）。尽管LAD区域的损伤指数相似，但尤卡坦小型猪组与饲养猪组相比，NI/M比值有升高趋势（p = 0.07；方差分析）（分别为1.14±0.21和0.73±0.09，每组n = 7）。在LCX区域我们未发现这种趋势，该区域的损伤指数（25.7%±3.5%）显著高于LAD区域（18.2%±1.2%，p < 0.05）。如果考虑到球囊诱导的血管损伤的差异，冠状动脉球囊过度充盈技术在成年或幼年猪中测试潜在的抗再狭窄药物时应会证明是有用的。
