Vever-Bizet C, Brault D
Laboratoire de Biophysique, INSERM U. 201, CNRS U.A. 481, Muséum National d'Histoire Naturelle, Paris, France.
Biochim Biophys Acta. 1993 Dec 12;1153(2):170-4. doi: 10.1016/0005-2736(93)90402-l.
The kinetics of hematoporphyrin or deuteroporphyrin incorporation in egg phosphatidylcholine small unilamellar vesicles have been investigated by fluorescence stopped-flow measurements. The processes can be described by a fast equilibrium. The on-rate constant is nearly diffusion controlled regardless of the compound used and the pH. The affinity of these porphyrins for the vesicles is merely governed by the exit rate which depends on the structure of the porphyrin and on its charge determined by pH.
通过荧光停流测量研究了血卟啉或次卟啉掺入卵磷脂小单层囊泡的动力学。这些过程可用快速平衡来描述。无论使用何种化合物和pH值，结合速率常数几乎受扩散控制。这些卟啉对囊泡的亲和力仅由逸出速率决定，而逸出速率取决于卟啉的结构及其由pH值决定的电荷。
