单个钠离子通道分子两种构象转变的热力学熵
Thermodynamic entropy of two conformational transitions of single Na+ channel molecules.
作者信息
Benndorf K, Koopmann R
机构信息
Department of Physiology, University of Cologne, Germany.
出版信息
Biophys J. 1993 Oct;65(4):1585-9. doi: 10.1016/S0006-3495(93)81197-4.
Abstract
Single cardiac Na+ channel currents were recorded with improved resolution (bandwidth up to 20 kHz) at two temperatures, 10 and 25 degrees C. The mean open time was determined at voltages between -50 and 0 mV by evaluation of the distribution of the event-related gaps in the center of the baseline noise. Fit of the voltage-dependent reciprocal mean open times at both temperatures allowed even for a single channel molecule to separate an entropic from an enthalpic part of activation energy for both deactivation and inactivation. Both entropies are positive and the entropy of deactivation exceeds that of inactivation by more than twice.
摘要
在10摄氏度和25摄氏度这两个温度下，以更高的分辨率（带宽高达20千赫兹）记录了单个心脏钠通道电流。通过评估基线噪声中心与事件相关的间隙分布，在-50至0毫伏的电压下确定了平均开放时间。对两个温度下电压依赖性倒数平均开放时间的拟合，甚至允许单个通道分子区分失活和失活的活化能的熵部分和焓部分。两个熵都是正的，失活的熵超过失活的熵两倍以上。
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