酵母自主复制序列元件的结构与功能。

The structure and function of yeast ARS elements.

作者信息

Newlon C S, Theis J F

机构信息

Department of Microbiology and Molecular Genetics, UMD-New Jersey Medical School, Newark 07103.

出版信息

Curr Opin Genet Dev. 1993 Oct;3(5):752-8. doi: 10.1016/s0959-437x(05)80094-2.

DOI:10.1016/s0959-437x(05)80094-2

PMID:8274858

Abstract

The past year has seen significant advances in our understanding of the structure and function of yeast ARS elements. These elements, some of which function as chromosomal origins of DNA replication, are modular in structure. An essential domain, the ARS consensus sequence, binds a multiprotein complex that might be the long-sought initiator protein. The flanking domain contains a DNA unwinding element and a binding site for a multifunctional protein that acts as a replication enhancer.

摘要

在过去的一年里，我们对酵母自主复制序列（ARS）元件的结构和功能的理解取得了重大进展。这些元件中的一些作为DNA复制的染色体起源发挥作用，其结构是模块化的。一个必需结构域，即ARS共有序列，结合一个多蛋白复合体，该复合体可能就是长期以来寻找的起始蛋白。侧翼结构域包含一个DNA解旋元件和一个多功能蛋白的结合位点，该多功能蛋白作为复制增强子发挥作用。