过滤灭菌和加热灭菌的脑心浸液血琼脂对淋病奈瑟菌生长影响的比较

A comparison of brain heart infusion blood agar sterilized by filtration and heat on the growth of Neisseria gonorrhoeae.

作者信息

Seth A D, Wilkinson A E

出版信息

J Clin Pathol. 1976 Dec;29(12):1091-3. doi: 10.1136/jcp.29.12.1091.

DOI:10.1136/jcp.29.12.1091

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC476308/

Abstract

The growth of Neisseria gonorrhoeae on brain heart infusion blood agar in which the base was sterilized by filtration has been compared with growth on the same medium sterilized by heat. Colonies were larger on the unheated medium, and autoclaving at 115 degrees C of 121 degrees C for 15 minutes was accompanied by a progressive decrease in colony size. Viable counts on the three media showed no difference in end points. Colonies on the unheated medium were usually large enough to be easily recognizable after overnight incubation.

摘要

将淋病奈瑟菌在通过过滤灭菌的脑心浸液血琼脂上的生长情况，与在经加热灭菌的相同培养基上的生长情况进行了比较。未加热的培养基上菌落更大，在115℃或121℃下高压灭菌15分钟会伴随着菌落大小逐渐减小。三种培养基上的活菌计数在终点时没有差异。未加热培养基上的菌落通常足够大，过夜培养后很容易识别。

相似文献

1

A comparison of brain heart infusion blood agar sterilized by filtration and heat on the growth of Neisseria gonorrhoeae.

J Clin Pathol. 1976 Dec;29(12):1091-3. doi: 10.1136/jcp.29.12.1091.

2

Suitability of a chocolate agar-based medium for the transportation and long term storage of Neisseria gonorrhoeae isolates at room temperature.

J Microbiol Methods. 2018 Jan;144:86-87. doi: 10.1016/j.mimet.2017.11.011. Epub 2017 Nov 15.

3

Simplified complete medium for the growth of Neisseria gonorrhoeae.

J Clin Microbiol. 1977 Jan;5(1):9-14. doi: 10.1128/jcm.5.1.9-14.1977.

4

Cultivation and properties of Neisseria sp. grown in chemically defined media.

Can J Microbiol. 1972 Jul;18(7):1087-90. doi: 10.1139/m72-168.

5

Effect of culture-medium temperature on growth of Neisseria gonorrhoeae.

J Am Osteopath Assoc. 1990 Aug;90(8):683-5.

6

Autoclaving: a modification in the preparation of tissue culture medium 199.

Can J Microbiol. 1972 Feb;18(2):272-3. doi: 10.1139/m72-042.

7

Modified transport and growth medium for the cultivation of Neisseria gonorrhoeae.

Br J Vener Dis. 1974 Jun;50(3):199-201. doi: 10.1136/sti.50.3.199.

8

[Evaluation of different methods of utilization of sugars in Neisseria gonorrhoeae].

Rev Cubana Med Trop. 2003 May-Aug;55(2):88-90.

9

Laboratory and clinical evaluation of modified New York City medium (Henderson formulation) for the isolation of Neisseria gonorrhoeae.

J Clin Microbiol. 1984 Nov;20(5):905-8. doi: 10.1128/jcm.20.5.905-908.1984.

10

Colony formation by sublethally heat-injured Zygosaccharomyces rouxii as affected by solutes in the recovery medium and procedure for sterilizing medium.

Appl Environ Microbiol. 1990 Aug;56(8):2319-26. doi: 10.1128/aem.56.8.2319-2326.1990.

本文引用的文献

1

A SELECTIVE MEDIUM FOR THE CULTIVATION OF N. GONORRHOEAE AND N. MENINGITIDIS.

Public Health Rep (1896). 1964 Jan;79(1):49-57.

2

Fluorescent antibody methods for Neisseria gonorrhoea identification.

Bull World Health Organ. 1961;24(3):349-55.

3

Slide agglutination method for the serological identification of Neisseria gonorrhoeae with anti-gonococcal antibodies adsorbed to protein A-containing staphylococci.

Appl Microbiol. 1974 Feb;27(2):368-74. doi: 10.1128/am.27.2.368-374.1974.

4

Some observations on cooling in laboratory autoclaves.

J Clin Pathol. 1975 Aug;28(8):664-9. doi: 10.1136/jcp.28.8.664.

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍。

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

文档翻译

学术文献翻译模型，支持多种主流文档格式。