Rafael Llanes, Sánchez Idalmis, Díaz Joel, Gutiérrez Oderay, Guzmán Daymi, Sosa Jorge, Valdés Eduardo A
Instituto de Medicina Tropical "Pedro Kourí", Ciudad de La Habana, Cuba.
Rev Cubana Med Trop. 2003 May-Aug;55(2):88-90.
5 methods of utilization of sugars were evaluated in 25 strains previously identified as N. gonorrhoeae: CTA agar, modified CTA agar, gelatin starch agar, Mueller Hinton agar plus bromotimol blue and rapid method. 100% of the strains of N. gonorrhoeae were identified by the CTA and rapid methods, whereas 96% were identified by the modified CTA and gelatin starch agar methods. No strain of gonoccocus was identified by the Mueller Hinton agar method plus bromotimol blue. The cystine tripticase agar medium (CTA) is the elective method to confirm the isolates of Neisseria gonorrhoeae. As it is mainly made by U.S. companies, it is difficult for Cuba to acquire it. The gelatin-starch agar method and the rapid method are useful alternative of the CTA medium, so their use is proposed in this paper.
在先前鉴定为淋病奈瑟菌的25株菌株中评估了5种糖利用方法:CTA琼脂、改良CTA琼脂、明胶淀粉琼脂、添加溴百里酚蓝的穆勒-欣顿琼脂和快速方法。100%的淋病奈瑟菌菌株通过CTA和快速方法鉴定出来,而96%通过改良CTA和明胶淀粉琼脂方法鉴定出来。添加溴百里酚蓝的穆勒-欣顿琼脂方法未鉴定出任何淋病奈瑟菌菌株。胱氨酸胰蛋白酶琼脂培养基(CTA)是确认淋病奈瑟菌分离株的选择方法。由于它主要由美国公司生产,古巴很难获得。明胶淀粉琼脂方法和快速方法是CTA培养基的有用替代方法,因此本文建议使用它们。