Demetrick D J, Beach D H
Cold Spring Harbor Laboratory, New York 11724.
Genomics. 1993 Oct;18(1):144-7. doi: 10.1006/geno.1993.1440.
The human CDC25 tyrosine phosphatases trigger activation of CDC2 by removing inhibitory phosphates; thus the genes encoding these phosphatases may be suspected as potential oncogenes due to their role in promoting cell division. To date, three human CDC25 genes have been identified: CDC25A, B, and C. This communication describes the mapping of CDC25A to chromosome 3p21 and CDC25B to chromosome 20p13 by fluorescence in situ hybridization with confirmation by the polymerase chain reaction of hamster-human somatic cell hybrid DNA. 3p21 is near an area frequently involved in karyotypic abnormalities in renal carcinomas, small cell carcinomas of the lung, and benign tumors of the salivary gland. 20p13 does not seem to be a common area for karyotypic alteration in tumors. Mapping of these genes to their chromosomal loci may help identify tumors with abnormal regulation of CDC25 genes due to genomic alterations.
人类CDC25酪氨酸磷酸酶通过去除抑制性磷酸基团来触发CDC2的激活；因此，编码这些磷酸酶的基因可能因其在促进细胞分裂中的作用而被怀疑是潜在的癌基因。到目前为止，已鉴定出三种人类CDC25基因：CDC25A、B和C。本通讯描述了通过荧光原位杂交将CDC25A定位于染色体3p21，将CDC25B定位于染色体20p13，并通过仓鼠-人类体细胞杂种DNA的聚合酶链反应进行了确认。3p21靠近一个在肾癌、肺小细胞癌和唾液腺良性肿瘤中经常涉及核型异常的区域。20p13似乎不是肿瘤核型改变的常见区域。将这些基因定位于其染色体位点可能有助于识别由于基因组改变而导致CDC25基因调控异常的肿瘤。
