从人类21号染色体分离并鉴定14个CA重复微卫星序列。

Isolation and characterization of 14 CA-repeat microsatellites from human chromosome 21.

作者信息

Bosch A, Nunes V, Patterson D, Estivill X

机构信息

Molecular Genetics Department, Hospital Duran i Reynals, Barcelona, Catalunya, Spain.

出版信息

Genomics. 1993 Oct;18(1):151-5. doi: 10.1006/geno.1993.1442.

DOI:10.1006/geno.1993.1442

PMID:8276404

Abstract

Fourteen CA-repeat microsatellite polymorphisms from human chromosome 21 (HC21) (D21S364, D21S365, D21S366, D21S367, D21S368, D21S369, D21S406, D21S407, D21S408, D21S409, D21S410, D21S411, D21S415, and D21S1217) have been cloned from a flow-sorted phage library (LA21NS01). These microsatellite loci have been mapped using a somatic cell hybrid panel of HC21, and heterozygosities and allele frequencies have been determined. The markers spread along the length of the long arm of the HC21. Twelve of these microsatellites have heterozygosities between 0.47 and 0.90. These new CA-repeat markers will improve the genetic map of HC21.

摘要

从人21号染色体（HC21）上克隆了14个CA重复微卫星多态性位点（D21S364、D21S365、D21S366、D21S367、D21S368、D21S369、D21S406、D21S407、D21S408、D21S409、D21S410、D21S411、D21S415和D21S1217），来自一个流式分选的噬菌体文库（LA21NS01）。这些微卫星位点已通过HC21的体细胞杂种板进行定位，并确定了杂合度和等位基因频率。这些标记沿着HC21长臂的长度分布。其中12个微卫星的杂合度在0.47至0.90之间。这些新的CA重复标记将改善HC21的遗传图谱。