Nakabo Y, Harakawa N, Yamamoto K, Okuma M, Uno K, Sasada M
Department of Internal Medicine, Faculty of Medicine, Kyoto University.
Jpn J Cancer Res. 1993 Nov;84(11):1174-80. doi: 10.1111/j.1349-7006.1993.tb02818.x.
In this study, we analyzed the mechanism(s) of leukemic cell lysis by human macrophages. Peripheral blood monocyte-derived macrophages were activated with recombinant interferon-gamma and lipopolysaccharide and their lytic activity against two leukemic cell lines (K562 and HL-60 cells) was assessed by an 111In releasing assay. Activated macrophages lysed these leukemic cells, and the lytic activity against leukemic cells was almost completely inhibited by anti-tumor necrosis factor (TNF) antibody. The macrophage-lysate prepared from activated macrophages also exhibited significant lytic activity against leukemic cells; this lytic activity was inhibited by anti-TNF antibody. The leukemic cells that we used for the cytotoxicity assays were resistant to recombinant TNF. The culture supernatant of activated macrophages did not show any lytic activity. These findings suggest that cell-associated TNF plays a role in macrophage-mediated cytotoxicity against leukemic cells.
在本研究中，我们分析了人类巨噬细胞对白血病细胞的裂解机制。用重组干扰素-γ和脂多糖激活外周血单核细胞衍生的巨噬细胞，并通过铟-111释放试验评估它们对两种白血病细胞系（K562和HL-60细胞）的裂解活性。活化的巨噬细胞裂解这些白血病细胞，并且抗肿瘤坏死因子（TNF）抗体几乎完全抑制了对白血病细胞的裂解活性。从活化巨噬细胞制备的巨噬细胞裂解物也表现出对白血病细胞的显著裂解活性；这种裂解活性被抗TNF抗体抑制。我们用于细胞毒性试验的白血病细胞对重组TNF具有抗性。活化巨噬细胞的培养上清液未显示任何裂解活性。这些发现表明，细胞相关的TNF在巨噬细胞介导的对白血病细胞的细胞毒性中起作用。
