Fidler I J, Kleinerman E S
J Clin Oncol. 1984 Aug;2(8):937-43. doi: 10.1200/JCO.1984.2.8.937.
The possibility that tumoricidal human blood monocytes would recognize and destroy tumorigenic targets but leave bystander nontumorigenic cells unharmed was investigated. Highly purified preparations of peripheral blood monocytes isolated from normal human donors were activated in vitro by incubation with human lymphokines encapsulated in multilamellar liposomes. The cytotoxic properties of these monocytes against several tumorigenic and nontumorigenic allogeneic target cell populations were assessed by an in vitro radioisotope-release assay. Various combinations of three tumorigenic and three nontumorigenic target-cell populations, labeled with either [3H]thymidine or [14C]thymidine, were mixed and plated onto monolayers of blood monocytes. In all combinations used, activated monocytes specifically lysed only allogeneic neoplastic cells. At least in vitro, activated human blood monocytes can recognize and selectively destroy neoplastic cells.
研究了具有杀肿瘤作用的人血单核细胞识别并破坏致瘤靶标，但使旁观者非致瘤细胞不受伤害的可能性。从正常人类供体分离的外周血单核细胞的高度纯化制剂，通过与包裹在多层脂质体中的人淋巴因子一起孵育在体外被激活。通过体外放射性同位素释放试验评估这些单核细胞对几种致瘤和非致瘤同种异体靶细胞群体的细胞毒性特性。将用[3H]胸腺嘧啶核苷或[14C]胸腺嘧啶核苷标记的三种致瘤和三种非致瘤靶细胞群体的各种组合混合，并接种到血单核细胞单层上。在所有使用的组合中，活化的单核细胞仅特异性裂解同种异体肿瘤细胞。至少在体外，活化的人血单核细胞可以识别并选择性破坏肿瘤细胞。
