Himanen J P, Pyhälä L, Olander R M, Merimskaya O, Kuzina T, Lysyuk O, Pronin A, Sanin A, Helander I M, Sarvas M
National Public Health Institute, Helsinki, Finland.
J Gen Microbiol. 1993 Nov;139(11):2659-65. doi: 10.1099/00221287-139-11-2659.
To evaluate the suitability of Bacillus subtilis as a production host of heterologous proteins for pharmaceutical purposes, we assessed the biological activity of this bacterium and its major cell envelope components, lipoteichoic acid (LTA) and peptidoglycan-teichoic acid complex (PG-TA) in several eukaryotic effector assays. LTA and PG-TA were found to be non-toxic for mice and guinea-pigs in a short-term toxicity assay. PG-TA was weakly pyrogenic and weakly mitogenic. Both LTA and PG-TA acted as immunologic adjuvants in mice and when injected in mice, also caused an increase in the number of granulocyte-monocyte colony-forming cells in the bone marrow probably via stimulation of production of granulocyte-macrophage colony-stimulating factor.
为了评估枯草芽孢杆菌作为药用异源蛋白生产宿主的适用性，我们在几种真核效应检测中评估了该细菌及其主要细胞包膜成分脂磷壁酸（LTA）和肽聚糖-磷壁酸复合物（PG-TA）的生物活性。在短期毒性检测中，发现LTA和PG-TA对小鼠和豚鼠无毒。PG-TA有微弱的致热原性和微弱的促有丝分裂作用。LTA和PG-TA在小鼠中均作为免疫佐剂起作用，当注射到小鼠体内时，可能通过刺激粒细胞-巨噬细胞集落刺激因子的产生，也导致骨髓中粒细胞-单核细胞集落形成细胞数量增加。
