Paszkowski U, Zhang S B, Potrykus I, Paszkowski J
Institute for Plant Sciences, Swiss Federal Institute of Technology, ETH-Zurich.
J Gen Virol. 1993 Dec;74 ( Pt 12):2725-9. doi: 10.1099/0022-1317-74-12-2725.
The capacity for autonomous replication of the DNA A of African cassava mosaic virus (ACMV), a member of the bipartite geminiviruses infecting dicotyledonous plants, has been compared in host and non-host cells. A derivative of the ACMV DNA A was transfected into tobacco and maize protoplasts. Although ACMV is not able to infect maize, replication of the DNA A in maize protoplasts was observed to occur. The efficiency of replication was 10 to 20% of that seen in tobacco protoplasts. In both plant systems, replication was detected after the onset of cell division. ACMV replication in maize cells was compared to that of wheat dwarf virus and found to be 10 to 20% of that observed with the monocotyledon-specific virus. Insertion of 1165 bp of non-viral DNA into the ACMV DNA A prevented replication in maize but not in tobacco.
作为双分体双生病毒的成员之一，非洲木薯花叶病毒（ACMV）可感染双子叶植物，其DNA A的自主复制能力已在宿主细胞和非宿主细胞中进行了比较。将ACMV DNA A的一个衍生物转染到烟草和玉米原生质体中。尽管ACMV无法感染玉米，但观察到DNA A在玉米原生质体中发生了复制。复制效率为烟草原生质体中的10%至20%。在这两种植物系统中，细胞分裂开始后都检测到了复制。将ACMV在玉米细胞中的复制与小麦矮缩病毒的复制进行比较，发现其复制量为单子叶特异性病毒的10%至20%。在ACMV DNA A中插入1165 bp的非病毒DNA可阻止其在玉米中复制，但在烟草中则不然。
