Calcutt N A, Mizisin A P, Yaksh T L
Department of Pathology, University of California San Diego, La Jolla 92093.
J Neurol Sci. 1993 Nov;119(2):154-61. doi: 10.1016/0022-510x(93)90128-l.
This study was designed to assess the effects of experimental diabetes and nerve crush injury upon vasoactive intestinal polypeptide (VIP) content and axonal transport in the dorsal root ganglia (DRG) and sciatic nerve. Sciatic nerve crush injury in control and 3-week streptozotocin-diabetic rats was followed 6.5 days later by placement of 2 constricting ligatures above the site of injury. After 12 h, the L4 and L5 DRG and sciatic nerve were removed for VIP radioimmunoassay. Similar samples were also taken from control and diabetic rats whose nerve had been ligated without a preceding crush. VIP was increased over 2-fold in ganglia and 4-fold in nerves of crush-injured controls compared to uninjured controls (both P < 0.01). Crush injury also increased ganglion and nerve VIP in diabetic rats (P < 0.05 and 0.01, respectively) but the increase was less than what occurred in crush-injured controls (both P < 0.05). The accumulation of VIP proximal to a sciatic ligature was similar in control and diabetic rats and was not altered in either group by crush injury, while retrograde transport of VIP was initiated by crush injury in both control and diabetic rats. These data show that short-term diabetes does not alter the amount and peripheral axonal transport of VIP in the sciatic nerve but impairs the ability of peripheral nerve to respond to injury.
本研究旨在评估实验性糖尿病和神经挤压伤对背根神经节（DRG）和坐骨神经中血管活性肠肽（VIP）含量及轴突运输的影响。在对照大鼠和3周链脲佐菌素诱导的糖尿病大鼠中进行坐骨神经挤压伤，6.5天后在损伤部位上方放置2个缩窄结扎线。12小时后，取出L4和L5背根神经节及坐骨神经进行VIP放射免疫测定。还从神经已结扎但未预先挤压的对照大鼠和糖尿病大鼠中采集了类似样本。与未受伤的对照相比，挤压伤对照的神经节中VIP增加了2倍以上，神经中增加了4倍（均P < 0.01）。挤压伤也增加了糖尿病大鼠神经节和神经中的VIP（分别为P < 0.05和0.01），但增加幅度小于挤压伤对照（均P < 0.05）。坐骨神经结扎近端VIP的积累在对照大鼠和糖尿病大鼠中相似，且两组中挤压伤均未改变，而VIP的逆行运输在对照大鼠和糖尿病大鼠中均由挤压伤启动。这些数据表明，短期糖尿病不会改变坐骨神经中VIP的含量和外周轴突运输，但会损害外周神经对损伤的反应能力。
