Van Dekken H, Bosman F T, Teijgeman R, Vissers C J, Tersteeg T A, Kerstens H M, Vooijs G P, Verhofstad A A
Department of Pathology, Erasmus University Rotterdam, The Netherlands.
J Pathol. 1993 Nov;171(3):161-71. doi: 10.1002/path.1711710304.
We have applied non-isotopic in situ hybridization (ISH) to interphase cell nuclei of 23 phaeochromocytomas (18 primary and 5 metastatic tumours) within routine paraffin-embedded tissue sections. Each tumour was screened for numerical aberrations with a defined alphoid repetitive DNA probe set containing DNA probes specific for chromosomes 1, 7, 15, and Y. Normal adrenal medullas and other normal human cell types served as cytogenetic controls. Preservation of tissue morphology enabled targeted analysis of tumour cells. The presence of numerical chromosome changes in the tumour cells could easily be evaluated by comparing the ISH results of the DNA probes. Numerical abnormalities not previously reported in this neoplasm included overrepresentation of chromosomes 1 and 7, loss of chromosome 15, and both gain and loss of chromosome Y (P values < 0.01). The percentage of aneuploid cell nuclei in a tumour correlated well with the percentage of cells in the 4C peak of flow cytometric DNA histograms from these neoplasms. We conclude that interphase ISH can be used for the identification of new and reported cytogenetic changes in tumour cell nuclei within archival tissue sections. This novel procedure also allows for retrospective analysis of previously not karyotyped material.
我们已将非同位素原位杂交（ISH）应用于常规石蜡包埋组织切片中23例嗜铬细胞瘤（18例原发性肿瘤和5例转移性肿瘤）的间期细胞核。使用一组特定的α卫星重复DNA探针（包含针对1号、7号、15号染色体及Y染色体的特异性DNA探针）对每个肿瘤进行染色体数目异常筛查。正常肾上腺髓质及其他正常人类细胞类型作为细胞遗传学对照。组织形态的保存使得能够对肿瘤细胞进行靶向分析。通过比较DNA探针的ISH结果，可轻松评估肿瘤细胞中染色体数目的变化。该肿瘤此前未报道的染色体数目异常包括1号和7号染色体的过度表达、15号染色体的缺失以及Y染色体的增减（P值<0.01）。肿瘤中非整倍体细胞核的百分比与这些肿瘤流式细胞术DNA直方图中4C峰的细胞百分比密切相关。我们得出结论，间期ISH可用于识别存档组织切片中肿瘤细胞核新的和已报道的细胞遗传学变化。这一新技术还允许对以前未进行核型分析的材料进行回顾性分析。
