Uchida K
Institute of Basic Medical Sciences, University of Tsukuba.
Nihon Rinsho. 1993 Nov;51(11):3051-61.
ADP-ribosylation is a post-translational modification of protein using a respiratory coenzyme, NAD+, as a substrate. Poly(ADP-ribosyl)ation reaction in the nuclei has been thought to be involved in DNA repair. Cloning of poly(ADP-ribose)polymerase cDNA and gene has opened the way for analyzing the structure and functions of poly(ADP-ribose)polymerase at the molecular level. Poly(ADP-ribose)polymerase is a zinc-finger protein and binds to nicks of DNA. This enzyme is activated by binding to DNA and poly(ADP-ribosyl)ates nuclear proteins and poly(ADP-ribose)polymerase itself. Structural analysis of poly(ADP-ribose)polymerase from different classes has shown conserved motifs in the domain structures. In this review, recent advances in this field, including mono-ADP-ribosylation and cyclic ADP-ribose, are described.
ADP核糖基化是一种蛋白质的翻译后修饰，它以呼吸辅酶NAD⁺作为底物。细胞核中的聚（ADP-核糖）化反应被认为与DNA修复有关。聚（ADP-核糖）聚合酶cDNA和基因的克隆为在分子水平上分析聚（ADP-核糖）聚合酶的结构和功能开辟了道路。聚（ADP-核糖）聚合酶是一种锌指蛋白，可与DNA的切口结合。该酶通过与DNA结合而被激活，并对核蛋白和聚（ADP-核糖）聚合酶自身进行聚（ADP-核糖）化。对不同类别的聚（ADP-核糖）聚合酶的结构分析表明，其结构域结构中存在保守基序。在这篇综述中，将描述该领域的最新进展，包括单ADP核糖基化和环状ADP核糖。
