一种用于监测啮齿动物群体中念珠状链杆菌抗体的酶联免疫吸附测定（ELISA）。

An enzyme-linked immunosorbent assay (ELISA) for monitoring rodent colonies for Streptobacillus moniliformis antibodies.

作者信息

Boot R, Bakker R H, Thuis H, Veenema J L, De Hoog H

机构信息

Department of Laboratory Animal Microbiology, National Institute of Public Health and Environmental Protection, Bilthoven, The Netherlands.

出版信息

Lab Anim. 1993 Oct;27(4):350-7. doi: 10.1258/002367793780745516.

DOI:10.1258/002367793780745516

PMID:8277708

Abstract

An enzyme-linked immunosorbent assay (ELISA) to measure Streptobacillus moniliformis antibodies in mice and rats was developed. Twelve S. moniliformis strains originating from cases of rat-bite fever and Haverhill fever in man and from various rodent species, showed considerable serological relationship. The ELISA appeared specific since antibodies to S. moniliformis were absorbed by autologous and homologous antigen, but not by heterologous bacterial antigens. Acholeplasma laidlawii showed partial serological relationship with S. moniliformis. The ELISA was validated using experimental infections in mice and rats. These studies and observations in naturally infected feral rats, confirmed that S. moniliformis is difficult to grow on primary isolation, and that the ELISA for S. moniliformis antibodies revealed more contaminated animals than culture.

摘要

开发了一种用于检测小鼠和大鼠中念珠状链杆菌抗体的酶联免疫吸附测定（ELISA）。来自人类鼠咬热和哈弗希尔热病例以及各种啮齿动物物种的12株念珠状链杆菌菌株显示出相当大的血清学关系。该ELISA似乎具有特异性，因为念珠状链杆菌的抗体可被自身和同源抗原吸收，但不能被异源细菌抗原吸收。莱氏无胆甾原体与念珠状链杆菌显示出部分血清学关系。通过对小鼠和大鼠进行实验性感染对该ELISA进行了验证。这些对自然感染的野生大鼠的研究和观察结果证实，念珠状链杆菌在初次分离时很难生长，并且用于检测念珠状链杆菌抗体的ELISA显示出比培养法更多的受污染动物。