Orgambide G G, Huang Z H, Gage D A, Dazzo F B
Department of Microbiology, Michigan State University, East Lansing 48824.
Lipids. 1993 Nov;28(11):975-9. doi: 10.1007/BF02537117.
The phospholipid and associated fatty acid compositions of the bacterial symbiont of clover, Rhizobium leguminosarum biovar trifolii wild-type ANU843, was analyzed by two-dimensional silica thin-layer chromatography, fast atom bombardment-mass spectrometry, flame-ionization detection gas-liquid chromatography and combined gas-liquid chromatography/mass spectrometry. The phospholipid composition included phosphatidylethanolamine (15%), N-methylphosphatidylethanolamine (47%), N,N-dimethylphosphatidylethanolamine (9%), phosphatidylglycerol (19%), cardiolipin (5%) and phosphatidylcholine (2%). Fatty acid composition included predominantly cis-11-octadecenoic acid, lower levels of cis-9-hexadecenoic acid, hexadecanoic acid, 11-methyl-11-octadecenoic acid, octadecanoic acid, 11,12-methyleneoctadecanoic acid, eicosanoic acid and traces of branched, and di- and triunsaturated fatty acids. The influence of expression of the "nodulation" genes encoding symbiotic functions on the composition of these membrane lipids was examined in wild-type cells grown with or without the flavone inducer, 4',7-dihydroxyflavone and in mutated cells lacking the entire symbiotic plasmid where these genes reside, or containing single transposon insertions in selected nodulation genes. No significant changes in phospholipid or associated fatty acid compositions were detected by the above methods of analysis.
采用二维硅胶薄层色谱法、快原子轰击质谱法、火焰离子化检测气液色谱法以及气液色谱/质谱联用分析法，对三叶草的细菌共生体——豌豆根瘤菌生物变种三叶草根瘤菌野生型ANU843的磷脂及相关脂肪酸组成进行了分析。磷脂组成包括磷脂酰乙醇胺（15%）、N-甲基磷脂酰乙醇胺（47%）、N,N-二甲基磷脂酰乙醇胺（9%）、磷脂酰甘油（19%）、心磷脂（5%）和磷脂酰胆碱（2%）。脂肪酸组成主要包括顺式-11-十八碳烯酸，含量较低的顺式-9-十六碳烯酸、十六烷酸、11-甲基-11-十八碳烯酸、十八烷酸、11,12-亚甲基十八烷酸、二十烷酸以及痕量的支链脂肪酸、二不饱和脂肪酸和三不饱和脂肪酸。在添加或不添加黄酮诱导剂4',7-二羟基黄酮的条件下培养野生型细胞，并在缺乏这些基因所在的整个共生质粒的突变细胞或在选定的结瘤基因中含有单个转座子插入的突变细胞中，研究了编码共生功能的“结瘤”基因表达对这些膜脂组成的影响。通过上述分析方法未检测到磷脂或相关脂肪酸组成有显著变化。
