White D C, Frerman F E
J Bacteriol. 1967 Dec;94(6):1854-67. doi: 10.1128/jb.94.6.1854-1867.1967.
Satisfactory extraction and assay procedures have been developed for the lipids of Staphylococcus aureus. The following lipids have been characterized in detail: the vitamin K(2), which is shown to exist as isoprenologues with side chains of 35, 40, and 45 carbon atoms; monoglucosyldiglyceride and diglucosyldiglyceride, which account for all the carbohydrate in the lipid extracts; the lysyl ester of phosphatidyl glycerol, phosphatidyl glycerol, and cardiolipin, which account for 98% of the phosphate in the lipid extract. The extraction procedure removes 98% of the total bacterial fatty acids. Acidification of the medium before harvest and refluxing in isopropanol are critical in the extraction procedure for the maximal recovery of lysyl-phosphatidyl glycerol and the glucolipids. The lipids have been shown to be a part of the same membrane as the respiratory pigments.
已开发出用于金黄色葡萄球菌脂质的满意提取和分析程序。已对以下脂质进行了详细表征：维生素K(2)，显示其以具有35、40和45个碳原子侧链的异戊二烯类似物形式存在；单葡萄糖二甘油酯和双葡萄糖二甘油酯，它们占脂质提取物中所有碳水化合物；磷脂酰甘油、磷脂酰甘油和心磷脂的赖氨酯，它们占脂质提取物中98%的磷酸盐。提取程序去除了98%的总细菌脂肪酸。收获前对培养基进行酸化并在异丙醇中回流，对于最大程度回收赖氨酰磷脂酰甘油和糖脂的提取程序至关重要。这些脂质已被证明是与呼吸色素相同膜的一部分。
