表明Cγ2同源区域不是一个规则结构域的证据。

Indications that the Cgamma2 homology region is not a regular domain.

作者信息

Michaelsen T E

出版信息

Scand J Immunol. 1976;5(10):1123-8. doi: 10.1111/j.1365-3083.1976.tb00253.x.

DOI:10.1111/j.1365-3083.1976.tb00253.x

Abstract

The Cgamma2 homology region of rabbit IgG does not behave like a domain. Thus, there is no trans-interaction between the two Cgamma2 regions; instead there is an unusual cis-interaction between Cgamma2 and Cgamma3 regions. The observations were made on the plasmin digestion products Facb (IgG minus the Cgamma3 region) and pFc' (Cgamma3 region), which did not dissociate under neutral conditions but dissociated in 3M guanidine solution (that is, cis-interaction between Cgamma2 and Cgamma3). The Facb fragment split into subunits with equal molecular weights under neutral conditions on partial reduction and alkylation (that is, lack of trans-interaction between the two Cgamma2 in the molecule).

摘要

兔IgG的Cγ2同源区的行为不像一个结构域。因此，两个Cγ2区域之间不存在反式相互作用；相反，Cγ2和Cγ3区域之间存在不寻常的顺式相互作用。这些观察结果是基于纤溶酶消化产物Facb（IgG减去Cγ3区域）和pFc′（Cγ3区域）得出的，它们在中性条件下不会解离，但在3M胍溶液中会解离（即Cγ2和Cγ3之间的顺式相互作用）。Facb片段在部分还原和烷基化后，在中性条件下会分裂成分子量相等的亚基（即分子中两个Cγ2之间缺乏反式相互作用）。