Merlo G R, Venesio T, Taverna D, Callahan R, Hynes N E
Friedrich Meischer Institute, Basel, Switzerland.
Ann N Y Acad Sci. 1993 Nov 30;698:108-13. doi: 10.1111/j.1749-6632.1993.tb17195.x.
We examined the status of the p53 gene in the HC11 normal mammary epithelial cells. Two mutations were identified: a Cys to Trp change at codon 138 and a microdeletion of codon 123 to 130 resulting from mutation of the splice acceptor site. These two mutations were independent, and no wild-type p53 allele was found. Introduction of wt-p53 strongly inhibited growth in monolayer. Thus, the absence of wt-p53 can be sufficient for the immortalization of mammary cells.
