兔乳腺中两种新型酪蛋白转录本的表征

Characterization of two novel casein transcripts in rabbit mammary gland.

作者信息

Dawson S P, Wilde C J, Tighe P J, Mayer R J

机构信息

Department of Biochemistry, University of Nottingham Medical School, Queens Medical Centre, U.K.

出版信息

Biochem J. 1993 Dec 15;296 ( Pt 3)(Pt 3):777-84. doi: 10.1042/bj2960777.

DOI:10.1042/bj2960777

PMID:8280077

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1137763/

Abstract

Two clones were isolated from a cDNA library corresponding to mRNAs which accumulate in mid-lactating (14 day) rabbit mammary gland and characterized by DNA sequencing. The two clones sequenced corresponded to two novel casein transcripts (pBRM5 and pBRM42). Relative mRNA abundances for the two clones were assessed by dot-blot analysis. Phylogenetic analysis and comparison of both pBRM5 and pBRM42 with other members of the casein family revealed that the rabbit may be unique among mammals in expressing two alpha s2-casein genes. The presence of two alpha s2-casein genes in the rabbit may be the result of a relatively recent intergenic duplication event.

摘要

从与在泌乳中期（14天）兔乳腺中积累的mRNA相对应的cDNA文库中分离出两个克隆，并通过DNA测序对其进行了表征。测序的两个克隆对应于两个新的酪蛋白转录本（pBRM5和pBRM42）。通过斑点印迹分析评估了这两个克隆的相对mRNA丰度。对pBRM5和pBRM42与酪蛋白家族其他成员进行系统发育分析和比较后发现，兔子在表达两个αs2-酪蛋白基因方面可能在哺乳动物中是独特的。兔子中两个αs2-酪蛋白基因的存在可能是相对近期的基因间重复事件的结果。