肝素作为一种控制核酸杂交程序背景的简单且经济高效手段的应用。
The use of heparin as a simple cost-effective means of controlling background in nucleic acid hybridization procedures.
作者信息
Singh L, Jones K W
出版信息
Nucleic Acids Res. 1984 Jul 25;12(14):5627-38. doi: 10.1093/nar/12.14.5627.
Abstract
The use of heparin as a simple, effective and cheap substitute for conventional methods of controlling background in hybridization procedures is described and illustrated with reference to the use of DNA probes in filter and in situ hybridization. Possible specific mechanisms for this heparin effect are discussed.
摘要
本文描述并举例说明了肝素作为一种简单、有效且廉价的替代品，用于替代杂交程序中控制背景的传统方法，具体涉及DNA探针在滤膜杂交和原位杂交中的应用。文中还讨论了肝素产生这种效应可能的具体机制。
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