Parent J L, Stankova J
Department of Pediatrics, Faculty of Medicine, University of Sherbrooke, Quebec, Canada.
Biochem Biophys Res Commun. 1993 Dec 30;197(3):1443-9. doi: 10.1006/bbrc.1993.2639.
Platelet-activating factor is a potent phospholipid mediator which produces a wide range of biological responses by binding to a specific, high-affinity receptor on the target cell surface. Platelet-activating factor has been shown to have a very important pro-inflammatory role while the cytokine transforming growth factor-beta, also known for its diverse and potent effects, can act as both a pro- and anti-inflammatory agent. To ascertain whether transforming growth factor-beta could modulate the expression of the human platelet-activating factor receptor, different cell types were stimulated with this cytokine and the effect determined by Northern blot analysis and Ca2+ influx studies. The results demonstrate that transforming growth factor-beta induces the expression of the platelet-activating factor receptor in monocytic and B cell lines and indicate that transforming growth factor-beta could play a role in the modulation of the inflammatory response by up-regulating the expression of the platelet-activating factor receptor.
血小板活化因子是一种强效磷脂介质，它通过与靶细胞表面的特异性高亲和力受体结合产生广泛的生物学反应。血小板活化因子已被证明具有非常重要的促炎作用，而细胞因子转化生长因子-β也因其多样而强效的作用而闻名，它既可以作为促炎剂，也可以作为抗炎剂。为了确定转化生长因子-β是否能调节人血小板活化因子受体的表达，用这种细胞因子刺激不同的细胞类型，并通过Northern印迹分析和Ca2+内流研究确定其作用效果。结果表明，转化生长因子-β可诱导单核细胞系和B细胞系中血小板活化因子受体的表达，并表明转化生长因子-β可能通过上调血小板活化因子受体的表达在炎症反应的调节中发挥作用。
