Nermut M V, Grief C, Hashmi S, Hockley D J
National Institute for Biological Standards and Control, Hertfordshire, England.
AIDS Res Hum Retroviruses. 1993 Oct;9(10):929-38. doi: 10.1089/aid.1993.9.929.
Specimens of HIV and SIV have been examined by electron microscopy, using the techniques of conventional thin sectioning, freeze-substitution, cryosectioning, and cryomicroscopy of frozen hydrated specimens. In addition freeze-drying and critical point drying were used for both shadowed replicas and scanning electron microscopy. Thin sections revealed hexagonal, pentagonal, or near-spherical profiles. Angular particles were seen in shadowed replicas and also by scanning electron microscopy. The images observed were consistent with an icosahedral shape of the virus. It is proposed that mature HIV (SIV) is an icosadeltahedron with flat triangular facets. Size measurements of the specimens showed a wide range of values for conventional embedding, but a narrow range for specimens prepared by low-temperature techniques.
已通过电子显微镜检查了HIV和SIV标本，采用了传统超薄切片、冷冻置换、冷冻切片以及冷冻水合标本的低温显微镜技术。此外，冷冻干燥和临界点干燥用于阴影复制品和扫描电子显微镜检查。超薄切片显示出六边形、五边形或近球形轮廓。在阴影复制品中以及通过扫描电子显微镜也可看到有角颗粒。观察到的图像与病毒的二十面体形状一致。有人提出成熟的HIV（SIV）是一个具有扁平三角形面的二十面体。标本的尺寸测量显示，传统包埋标本的值范围很广，但低温技术制备的标本值范围较窄。
