Inoue H, Ishii H, Tsutsumi M, Takahashi N, Sato M, Shimada Y, Kato T, Sudo T, Miyazaki H
Pharmaceutical Research Laboratory, Kirin Brewery Co. Ltd, Gunma, Japan.
Br J Haematol. 1993 Oct;85(2):260-9. doi: 10.1111/j.1365-2141.1993.tb03165.x.
The extended cytoplasmic processes from megakaryocytes (MK) are believed to be structural intermediates between MK and platelets. We could observe differentiation of purified rat CFU-MK toward mature MK to form extended cytoplasmic processes. Recombinant rat interleukin-3 (IL-3), human erythropoietin (Epo), and human interleukin-6 (IL-6) each was able to stimulate process formation although they varied somewhat in their potential. Electron microscopic observations showed that these processes were very similar to those from mature MK so far reported. The combination of IL-6 with IL-3 or Epo synergistically increased the number of MK forming processes without further increase in the number of total MK formed in the presence of IL-3 or Epo alone. In addition, IL-6 significantly increased the megakaryocytic diameter and DNA content of MK induced by IL-3 or Epo and shortened the MK transit time to hasten the process formation of MK. These findings suggest that IL-6 promotes further maturity in both the cytoplasm and ploidy of MK to form extended cytoplasmic processes. The ability of these factors to generate the process formation in vitro may be related to their thrombopoietic effects in vivo.
巨核细胞（MK）伸出的细胞质延长过程被认为是MK与血小板之间的结构中间体。我们能够观察到纯化的大鼠巨核细胞集落形成单位（CFU-MK）向成熟MK分化并形成延长的细胞质过程。重组大鼠白细胞介素-3（IL-3）、人促红细胞生成素（Epo）和人白细胞介素-6（IL-6）各自都能够刺激过程形成，尽管它们的潜力略有不同。电子显微镜观察表明，这些过程与迄今为止报道的成熟MK的过程非常相似。IL-6与IL-3或Epo联合使用可协同增加形成过程的MK数量，而在单独存在IL-3或Epo的情况下，形成的总MK数量不会进一步增加。此外，IL-6显著增加了由IL-3或Epo诱导的MK的巨核细胞直径和DNA含量，并缩短了MK的过渡时间，以加速MK的过程形成。这些发现表明，IL-6促进了MK细胞质和多倍体的进一步成熟，以形成延长的细胞质过程。这些因子在体外产生过程形成的能力可能与其在体内的血小板生成作用有关。
