An E, Ogata K, Kuriya S, Nomura T
Third Department of Internal Medicine, Nippon Medical School, Tokyo, Japan.
Exp Hematol. 1994 Feb;22(2):149-56.
Mouse megakaryocytes were purified using a rabbit antimouse platelet serum, and magnetic beads were conjugated with an antirabbit IgG antibody. The purified cells were 95.8 +/- 1.2% megakaryocytes, and the recovery and viability of the megakaryocytes were 70 +/- 18.4%, and 80 +/- 13.4%, respectively. The effects of recombinant erythropoietin (Epo), interleukin-6 (IL-6), and IL-1 beta on these purified megakaryocytes were studied. Epo and IL-6 significantly increased DNA synthesis in these cells, but IL-1 beta did not. Similarly, both Epo and IL-6, but not IL-1 beta, increased the acetylcholinesterase (AchE) activity in the megakaryocytes. Epo and IL-6 stimulated the megakaryocytes to form cytoplasmic processes, which are considered to represent in vitro proplatelet formation. This process formation was inhibited by the addition of colchicine to the cultures. It was concluded that Epo and IL-6 are not only direct potentiators of megakaryocytes, but also inducers of in vitro cytoplasmic process formation on megakaryocytes.
使用兔抗小鼠血小板血清纯化小鼠巨核细胞，并将磁珠与抗兔IgG抗体偶联。纯化后的细胞中巨核细胞占95.8±1.2%，巨核细胞的回收率和活力分别为70±18.4%和80±13.4%。研究了重组促红细胞生成素（Epo）、白细胞介素-6（IL-6）和白细胞介素-1β对这些纯化巨核细胞的影响。Epo和IL-6显著增加了这些细胞中的DNA合成，但IL-1β没有。同样，Epo和IL-6均增加了巨核细胞中的乙酰胆碱酯酶（AchE）活性，而IL-1β没有。Epo和IL-6刺激巨核细胞形成细胞质突起，这被认为代表体外前血小板形成。向培养物中添加秋水仙碱可抑制这种突起形成。得出的结论是，Epo和IL-6不仅是巨核细胞的直接增强剂，也是巨核细胞体外细胞质突起形成的诱导剂。
