Höfte H, Desprez T, Amselem J, Chiapello H, Rouzé P, Caboche M, Moisan A, Jourjon M F, Charpenteau J L, Berthomieu P
Laboratoire de Biologie Cellulaire, INRA, Versailles Cedex, France.
Plant J. 1993 Dec;4(6):1051-61. doi: 10.1046/j.1365-313x.1993.04061051.x.
As part of the goal to generate a detailed transcript map for Arabidopsis thaliana, 1152 single run sequences (expressed sequence tags or ESTs) have been determined from cDNA clones taken at random in libraries prepared from different sources of plant material: developing siliques, etiolated seedlings, flower buds, and cultured cells. Eight hundred and ninety-five different genes could be identified, 32% of which showed significant similarity to existing sequences in Arabidopsis and an array of other organisms. These sequences in combination with their positioning on the Arabidopsis genetic map will not only constitute a new set of molecular markers for genome analysis in Arabidopsis but also provide a direct route for the in vivo analysis of their gene products. The sequences have been made available to the public databases.
作为为拟南芥生成详细转录图谱目标的一部分,已从不同植物材料来源(发育中的角果、黄化幼苗、花芽和培养细胞)制备的文库中随机选取的 cDNA 克隆中确定了 1152 个单次运行序列(表达序列标签或 EST)。可以鉴定出 895 个不同的基因,其中 32% 与拟南芥及一系列其他生物体中的现有序列具有显著相似性。这些序列及其在拟南芥遗传图谱上的定位不仅将构成一组用于拟南芥基因组分析的新分子标记,还将为其基因产物的体内分析提供直接途径。这些序列已提交至公共数据库。