• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
Generation of expressed sequence tags of random root cDNA clones of Brassica napus by single-run partial sequencing.通过单次部分测序生成甘蓝型油菜随机根cDNA克隆的表达序列标签
Plant Physiol. 1993 Oct;103(2):359-70. doi: 10.1104/pp.103.2.359.
2
Classification and expression of a family of cyclin gene homologues in Brassica napus.甘蓝型油菜中一个细胞周期蛋白基因同源物家族的分类与表达
Plant Mol Biol. 1995 Jan;27(2):263-75. doi: 10.1007/BF00020182.
3
Cloning of PCP1, a member of a family of pollen coat protein (PCP) genes from Brassica oleracea encoding novel cysteine-rich proteins involved in pollen-stigma interactions.从甘蓝型油菜中克隆出PCP1，它是花粉壁蛋白（PCP）基因家族的一个成员，编码参与花粉与柱头相互作用的富含半胱氨酸的新型蛋白质。
Plant J. 1996 Aug;10(2):303-13. doi: 10.1046/j.1365-313x.1996.10020303.x.
4
Expressed sequence tags of Chinese cabbage flower bud cDNA.大白菜花芽cDNA的表达序列标签
Plant Physiol. 1996 Jun;111(2):577-88. doi: 10.1104/pp.111.2.577.
5
Evaluation of 515 expressed sequence tags obtained from guard cells of Brassica campestris.
Planta. 1997;202(1):9-17. doi: 10.1007/s004250050097.
6
Isolation and characterization of two Brassica napus embryo acyl-ACP thioesterase cDNA clones.两个甘蓝型油菜胚酰基-ACP硫酯酶cDNA克隆的分离与鉴定
Plant Mol Biol. 1993 Nov;23(4):769-78. doi: 10.1007/BF00021532.
7
Molecular analysis of two Brassica napus genes expressed in the stigma.对两个在柱头中表达的甘蓝型油菜基因的分子分析。
Plant Mol Biol. 1994 Nov;26(4):1217-22. doi: 10.1007/BF00040703.
8
Isolation of cDNA clones for genes that are expressed during leaf senescence in Brassica napus. Identification of a gene encoding a senescence-specific metallothionein-like protein.甘蓝型油菜叶片衰老过程中表达基因的cDNA克隆分离。一种编码衰老特异性金属硫蛋白样蛋白的基因的鉴定。
Plant Physiol. 1994 Jul;105(3):839-46. doi: 10.1104/pp.105.3.839.
9
Molecular characterization of two Brassica napus genes related to oleosins which are highly expressed in the tapetum.两个与油体蛋白相关的甘蓝型油菜基因的分子特征分析，这些基因在绒毡层中高度表达。
Plant J. 1994 Dec;6(6):927-33. doi: 10.1046/j.1365-313x.1994.6060927.x.
10
Characterization of three anther-specific genes isolated from Chinese cabbage.从大白菜中分离出的三个花药特异性基因的特征分析
Plant Mol Biol. 1997 Jan;33(1):193-8. doi: 10.1023/a:1005790832221.

引用本文的文献

1
Rapid and transient induction of calmodulin-encoding gene(s) of Brassica napus by a touch stimulus.油菜快速瞬时响应触刺激诱导钙调素编码基因的表达。
Plant Cell Rep. 1996 Apr;15(8):586-90. doi: 10.1007/BF00232458.
2
Computational analysis of common bean (Phaseolus vulgaris L., genotype BAT93) lycopene β-cyclase and β-carotene hydroxylase gene's cDNA.普通菜豆（菜豆属，基因型BAT93）番茄红素β-环化酶和β-胡萝卜素羟化酶基因cDNA的计算分析
Bioinformation. 2013;9(4):197-206. doi: 10.6026/97320630009197. Epub 2013 Feb 21.
3
Analysis of beta-carotene hydroxylase gene cDNA isolated from the American oil-palm (Elaeis oleifera) mesocarp tissue cDNA library.从美洲油棕（Elaeis oleifera）中果皮组织cDNA文库分离出的β-胡萝卜素羟化酶基因cDNA的分析。
Bioinformation. 2010 Sep 20;5(3):104-12. doi: 10.6026/97320630005104.
4
Comparative analysis of 5,211 leaf ESTs of wild rice (Oryza minuta).
Plant Cell Rep. 2004 Jun;22(11):839-47. doi: 10.1007/s00299-004-0764-4. Epub 2004 Feb 17.
5
Gene expression at early stages of Brassica napus seed development as revealed by transcript profiling of seed-abundant cDNAs.通过种子丰富cDNA的转录谱分析揭示的甘蓝型油菜种子发育早期的基因表达
Planta. 2004 Jan;218(3):483-91. doi: 10.1007/s00425-003-1124-2. Epub 2003 Oct 23.
6
Expressed Sequence Tags from Developing Castor Seeds.蓖麻籽发育过程中的表达序列标签
Plant Physiol. 1995 Jul;108(3):1141-1150. doi: 10.1104/pp.108.3.1141.
7
Dominant negative guard cell K+ channel mutants reduce inward-rectifying K+ currents and light-induced stomatal opening in arabidopsis.显性负性保卫细胞钾离子通道突变体降低拟南芥内向整流钾离子电流和光诱导的气孔开放。
Plant Physiol. 2001 Oct;127(2):473-85.
8
REP1, a basic helix-loop-helix protein, is required for a branch pathway of phytochrome A signaling in arabidopsis.REP1是一种碱性螺旋-环-螺旋蛋白，是拟南芥中光敏色素A信号传导分支途径所必需的。
Plant Cell. 2000 Nov;12(11):2061-74. doi: 10.1105/tpc.12.11.2061.
9
Expression analysis of ESts derived from the inner bark of Cryptomeria japonica.日本柳杉内皮来源的ESTs的表达分析。
Plant Mol Biol. 2000 Jul;43(4):451-7. doi: 10.1023/a:1006492103063.
10
Toward a functional catalog of the plant genome. A survey of genes for lipid biosynthesis.迈向植物基因组功能目录。脂质生物合成基因的调查。
Plant Physiol. 2000 Feb;122(2):389-402. doi: 10.1104/pp.122.2.389.

本文引用的文献

1
Primary Structure of Cytochrome b(5) from Cauliflower (Brassica oleracea L.) Deduced from Peptide and cDNA Sequences.从花椰菜（ Brassica oleracea L.）的肽和 cDNA 序列推导出细胞色素 b(5)的一级结构。
Plant Physiol. 1992 Jul;99(3):1254-7. doi: 10.1104/pp.99.3.1254.
2
Partial sequence analysis of 130 randomly selected maize cDNA clones.对130个随机选择的玉米cDNA克隆进行部分序列分析。
Plant Physiol. 1993 Jan;101(1):329-32. doi: 10.1104/pp.101.1.329.
3
Rapid searches for complex patterns in biological molecules.快速搜索生物分子中的复杂模式。
Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):263-80. doi: 10.1093/nar/12.1part1.263.
4
Turn prediction in proteins using a pattern-matching approach.使用模式匹配方法预测蛋白质中的转角。
Biochemistry. 1986 Jan 14;25(1):266-75. doi: 10.1021/bi00349a037.
5
Rapid and sensitive protein similarity searches.快速且灵敏的蛋白质相似性搜索。
Science. 1985 Mar 22;227(4693):1435-41. doi: 10.1126/science.2983426.
6
The gene family encoding the Arabidopsis thaliana translation elongation factor EF-1 alpha: molecular cloning, characterization and expression.编码拟南芥翻译延伸因子EF-1α的基因家族：分子克隆、特性及表达
Mol Gen Genet. 1989 Oct;219(1-2):106-12. doi: 10.1007/BF00261164.
7
Molecular cloning of the high affinity calcium-binding protein (calreticulin) of skeletal muscle sarcoplasmic reticulum.骨骼肌肌浆网高亲和力钙结合蛋白（钙网蛋白）的分子克隆
J Biol Chem. 1989 Dec 25;264(36):21522-8.
8
Multiple zones in the sequence of calreticulin (CRP55, calregulin, HACBP), a major calcium binding ER/SR protein.钙网蛋白（CRP55、钙调节蛋白、HACBP）序列中的多个区域，钙网蛋白是一种主要的钙结合内质网/肌浆网蛋白。
EMBO J. 1989 Dec 1;8(12):3581-6. doi: 10.1002/j.1460-2075.1989.tb08530.x.
9
Structural homology between the rat calreticulin gene product and the Onchocerca volvulus antigen Ral-1.大鼠钙网蛋白基因产物与旋盘尾丝虫抗原Ral-1之间的结构同源性。
Nucleic Acids Res. 1990 Aug 25;18(16):4933. doi: 10.1093/nar/18.16.4933.
10
An 'equalized cDNA library' by the reassociation of short double-stranded cDNAs.通过短双链cDNA重新缔合构建的“均一化cDNA文库”
Nucleic Acids Res. 1990 Oct 11;18(19):5705-11. doi: 10.1093/nar/18.19.5705.

通过单次部分测序生成甘蓝型油菜随机根cDNA克隆的表达序列标签

Generation of expressed sequence tags of random root cDNA clones of Brassica napus by single-run partial sequencing.

作者信息

Park Y S, Kwak J M, Kwon O Y, Kim Y S, Lee D S, Cho M J, Lee H H, Nam H G

机构信息

Department of Life Sciences, Pohang Institute of Science and Technology, Kyungbuk, South Korea.

出版信息

Plant Physiol. 1993 Oct;103(2):359-70. doi: 10.1104/pp.103.2.359.

DOI:10.1104/pp.103.2.359
PMID:8029332
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC158991/
Abstract

Two hundred thirty-seven expressed sequence tags (ESTs) of Brassica napus were generated by single-run partial sequencing of 197 random root cDNA clones. A computer search of these root ESTs revealed that 21 ESTs show significant similarity to the protein-coding sequences in the existing data bases, including five stress- or defense-related genes and four clones related to the genes from other kingdoms. Northern blot analysis of the 10 data base-matched cDNA clones revealed that many of the clones are expressed most abundantly in root but less abundantly in other organs. However, two clones were highly root specific. The results show that generation of the root ESTs by partial sequencing of random cDNA clones along with the expression analysis is an efficient approach to isolate genes that are functional in plant root in a large scale. We also discuss the results of the examination of cDNA libraries and sequencing methods suitable for this approach.

摘要

通过对197个随机根cDNA克隆进行单次部分测序，获得了237个甘蓝型油菜的表达序列标签（EST）。对这些根EST进行计算机搜索发现，21个EST与现有数据库中的蛋白质编码序列具有显著相似性，其中包括5个与胁迫或防御相关的基因以及4个与其他生物界基因相关的克隆。对10个与数据库匹配的cDNA克隆进行Northern印迹分析表明，许多克隆在根中表达最为丰富，而在其他器官中表达较少。然而，有两个克隆具有高度的根特异性。结果表明，通过对随机cDNA克隆进行部分测序以及表达分析来生成根EST，是大规模分离在植物根中起作用的基因的有效方法。我们还讨论了cDNA文库检查结果以及适用于该方法的测序方法。