Matsui H, Yamagata S, Hirano K, Ishikawa M, Fukutomi H, Osuga T
Department of Internal Medicine, University of Tsukuba, Ibaraki, Japan.
Dig Dis Sci. 1994 Jan;39(1):116-23. doi: 10.1007/BF02090070.
In an attempt to clarify whether gastric mucosal autofluorescence can help us to recognize gastric lesions at the onset of their formation, we investigated the fluorescence generated from the gastric mucosa of rats under ischemia-reperfusion stress. Red-colored fluorescence appeared and began to increase within 5 min after reperfusion. Such an increase in fluorescence did not occur in the gastric mucosa under prolonged ischemia without reperfusion. The epifluorescence microscopy of mucosal cryosections revealed that fluorescence was present even when only superficial mucosal damage occurred. Spectrofluorometric and high-performance liquid chromatographic analysis of the fluorescent mucosa extract identified the fluorescent substances as porphyrins. These findings suggested that fluorescent porphyrins are generated in the mucosal layer during the introductory phase of mucosal lesion formation induced by ischemia-reperfusion stress.
为了阐明胃黏膜自发荧光是否有助于我们在胃病变形成初期识别它们，我们研究了缺血再灌注应激下大鼠胃黏膜产生的荧光。再灌注后5分钟内出现红色荧光并开始增加。在长时间缺血而无再灌注的情况下，胃黏膜未出现这种荧光增加。黏膜冰冻切片的落射荧光显微镜检查显示，即使仅发生浅表黏膜损伤，也存在荧光。对荧光黏膜提取物进行荧光分光光度法和高效液相色谱分析，确定荧光物质为卟啉。这些发现表明，在缺血再灌注应激诱导的黏膜病变形成的初始阶段，黏膜层中会产生荧光卟啉。
