Fisher R L, Gandolfi A J, Sipes I G, Brendel K
University of Arizona, Department of Pharmacology, Tucson.
Drug Chem Toxicol. 1993;16(4):321-39. doi: 10.3109/01480549308998224.
The effects of different media composition on the hepatotoxicity produced by monochlorobenzene (MCB), 1,2-dichlorobenzene (1,2-DCB), 1,3-dichlorobenzene (1,3-DCB) and 1,4-dichlorobenzene (1,4-DCB) were examined in two different in vitro systems. The toxicity of these chlorobenzenes was investigated in the perfused rat liver and liver slices using Krebs-Henseleit buffer. Significant differences between the chlorobenzenes were apparent in the perfused liver but not in the tissue slices. However, a dose and time related response of rat liver slices to the chlorobenzenes was observed. Partial amelioration of the chlorobenzene toxicity was observed when the Krebs-Henseleit buffer was supplemented with vitamins, amino acids, and/or bovine serum albumin. 1,2-DCB and 1,3-DCB toxicity was affected by amino acids and vitamins. The toxicity produced by 1,4-DCB was suppressed by amino acids, vitamins and 1% BSA. MCB hepatoxicity could only be suppressed by 1% BSA. This data suggests that tissue culture media composition plays a major role in the hepatotoxicity of the chlorobenzenes.
在两种不同的体外系统中，研究了不同培养基成分对一氯苯（MCB）、1,2 - 二氯苯（1,2 - DCB）、1,3 - 二氯苯（1,3 - DCB）和1,4 - 二氯苯（1,4 - DCB）产生的肝毒性的影响。使用克雷布斯 - 亨泽莱特缓冲液，在灌注大鼠肝脏和肝切片中研究了这些氯苯的毒性。在灌注肝脏中，氯苯之间存在显著差异，但在组织切片中没有。然而，观察到大鼠肝切片对氯苯有剂量和时间相关的反应。当在克雷布斯 - 亨泽莱特缓冲液中补充维生素、氨基酸和/或牛血清白蛋白时，观察到氯苯毒性有部分改善。1,2 - DCB和1,3 - DCB的毒性受氨基酸和维生素的影响。1,4 - DCB产生的毒性被氨基酸、维生素和1%的牛血清白蛋白抑制。MCB的肝毒性仅被1%的牛血清白蛋白抑制。这些数据表明，组织培养基成分在氯苯的肝毒性中起主要作用。
