Hirouchi M, Hashimoto T, Kuriyama K
Department of Pharmacology, Kyoto Prefectural University of Medicine, Japan.
Eur J Pharmacol. 1993 Oct 15;247(2):127-30. doi: 10.1016/0922-4106(93)90069-l.
Alterations in the expression of mRNA for GABAA receptor alpha 1-subunit were analyzed in the brain using mice that had been made alcohol-dependent, and exhibited a decrease in GABA-dependent 36Cl- influx into membrane vesicles following continuous ethanol inhalation for 7 days. Continuous ethanol inhalation for more than 5 days induced a significant increase in the expression of GABAA receptor alpha 1-subunit mRNA in the brain without significantly altering total poly(A)+ RNA content. Furthermore, it was found that the increase in expression of GABAA receptor alpha 1-subunit mRNA in the brain turned to its normal level 8 h after the ethanol inhalation was terminated. In contrast, the expression of beta-actin mRNA in the brain was not altered under the same experimental conditions. These results suggest that continuous ethanol inhalation may induce not only the suppression of the functions of GABAA receptor complex but also a reactive increase in the expression of mRNA for GABAA receptor subunits in the brain.
利用已产生酒精依赖的小鼠，分析了大脑中GABAA受体α1亚基mRNA表达的变化。连续吸入乙醇7天后，这些小鼠膜囊泡中GABA依赖的36Cl-内流减少。连续吸入乙醇超过5天会导致大脑中GABAA受体α1亚基mRNA的表达显著增加，而总多聚腺苷酸加尾RNA（poly(A)+ RNA）含量没有明显改变。此外，还发现乙醇吸入终止8小时后，大脑中GABAA受体α1亚基mRNA的表达增加恢复到正常水平。相比之下，在相同实验条件下，大脑中β-肌动蛋白mRNA的表达没有改变。这些结果表明，连续吸入乙醇不仅可能导致GABAA受体复合物功能的抑制，还可能导致大脑中GABAA受体亚基mRNA表达的反应性增加。
