Mizoguchi T, Hayashida N, Yamaguchi-Shinozaki K, Kamada H, Shinozaki K
Laboratory of Plant Molecular Biology, Institute of Physical and Chemical Research (Riken), Ibaraki, Japan.
FEBS Lett. 1993 Dec 28;336(3):440-4. doi: 10.1016/0014-5793(93)80852-l.
We previously reported two cDNAs for MAP kinases (cATMPK1 and cATMPK2) from a dicot plant, Arabidopsis thaliana. We describe here the cloning and characterization of five additional cDNAs encoding novel MAP kinases in Arabidopsis, cATMPK3, cATMPK4, cATMPK5, cATMPK6, and cATMPK7. The amino acid residues corresponding to the sites of phosphorylation (Thr-Glu-Tyr) that are involved in the activation of animal MAP kinases are conserved in all the seven putative ATMPK proteins. Genes for MAP kinases in Arabidopsis constitute a family that contains more than seven members. Sequence analysis suggests that there are at least three subfamilies in the family of Arabidopsis genes for MAP kinases.
我们先前报道了来自双子叶植物拟南芥的两个促分裂原活化蛋白激酶（MAP激酶）的cDNA（cATMPK1和cATMPK2）。我们在此描述了拟南芥中另外五个编码新型MAP激酶的cDNA（cATMPK3、cATMPK4、cATMPK5、cATMPK6和cATMPK7）的克隆和特性分析。在所有七个推定的ATMPK蛋白中，与参与动物MAP激酶激活的磷酸化位点（苏氨酸-谷氨酸-酪氨酸）相对应的氨基酸残基是保守的。拟南芥中MAP激酶的基因构成了一个包含七个以上成员的家族。序列分析表明，拟南芥MAP激酶基因家族中至少有三个亚家族。
