鹿和绵羊胰岛素样生长因子-I及-II的分离与测序

Isolation and sequencing of deer and sheep insulin-like growth factors-I and -II.

作者信息

Moore L G, Jones D, Lymburn M A, Hodgkinson S C, Davis S R, Suttie J M, Sadighi M, Carne A

机构信息

Wallaceville Animal Research Centre, AgResearch, Upper Hutt, New Zealand.

出版信息

Gen Comp Endocrinol. 1993 Nov;92(2):302-10. doi: 10.1006/gcen.1993.1166.

DOI:10.1006/gcen.1993.1166

PMID:8282178

Abstract

We have developed a simple method for the isolation of highly purified cervine (c) and ovine (o) insulin-like growth factors-I (IGF-I) and -II. The IGFs were isolated from acidified serum by cation exchange chromatography and then purified by gel filtration, chromatofocusing, and reverse-phase chromatography. The IGF preparations are > 95% pure. The cIGF-I preparation contains < 0.056% cIGF-II and the oIGF-I preparation contains < 0.01% oIGF-II. Both the IGF-II preparations contain < 0.01% IGF-I. The amino acid sequence of cIGF-I has two differences when compared with human (h) IGF-I. The cIGF-II sequence, which is identical to bovine IGF-II, has three differences when compared with hIGF-II.

摘要

我们开发了一种简单的方法来分离高度纯化的鹿（c）和羊（o）胰岛素样生长因子-I（IGF-I）及-II。通过阳离子交换色谱从酸化血清中分离出IGF，然后通过凝胶过滤、色谱聚焦和反相色谱进行纯化。IGF制剂的纯度>95%。cIGF-I制剂中cIGF-II的含量<0.056%，oIGF-I制剂中oIGF-II的含量<0.01%。两种IGF-II制剂中IGF-I的含量均<0.01%。与人类（h）IGF-I相比，cIGF-I的氨基酸序列有两个差异。与牛IGF-II相同的cIGF-II序列，与hIGF-II相比有三个差异。