Bhagwat A A, Tully R E, Keister D L
Soybean and Alfalfa Research Laboratory, USDA, Agricultural Research Service, Beltsville, Maryland 20705.
FEMS Microbiol Lett. 1993 Dec 1;114(2):139-44. doi: 10.1111/j.1574-6968.1993.tb06564.x.
A cosmid clone complementing a cyclic beta-(1-->2)-glucan biosynthesis (ndvB) mutant of Rhizobium meliloti was isolated from a Bradyrhizobium japonicum gene library. This clone specified synthesis of beta-(1-->3), beta-(1-->6)-linked glucans in R. meliloti. The complemented strain was osmotically tolerant and symbiotically competent on alfalfa. Thus, beta-(1-->3), beta-(1-->6)-glucans can substitute functionally for beta-(1-->2)-glucans in R. meliloti.
从慢生根瘤菌基因文库中分离出一个能互补苜蓿根瘤菌环状β-(1→2)-葡聚糖生物合成(ndvB)突变体的黏粒克隆。该克隆在苜蓿根瘤菌中指定合成β-(1→3)、β-(1→6)连接的葡聚糖。互补菌株对渗透具有耐受性，并且在苜蓿上具有共生能力。因此，β-(1→3)、β-(1→6)葡聚糖在苜蓿根瘤菌中可以在功能上替代β-(1→2)葡聚糖。
