Eisert W G
Microsc Acta. 1976 Jul;78(3):228-37.
The laser flow microphotometer for rapid cell size analysis is introduced. In contrast to known principles absorption is detected of a part of each cell only. Cell classification is achieved by pulse form analysis of absorption pulses. Cell lengths between 5 and 300 mum have been measured without changing the geometry of the flow system. Other ranges of size can be covered by changing the flow diameters. The principle can be applied to stained or unstained cells. No further preparation of samples is necessary. Examples of high speed measurements on different cell populations of unstained ciliated (Tetrahymena pyriformis), human blood and different pollen grains are given.
介绍了用于快速细胞大小分析的激光流动显微光度计。与已知原理不同的是，它仅检测每个细胞的一部分的吸收情况。通过对吸收脉冲的脉冲形式分析来实现细胞分类。在不改变流动系统几何形状的情况下，已测量出细胞长度在5至300微米之间。通过改变流动直径可以覆盖其他大小范围。该原理可应用于染色或未染色的细胞。无需对样品进行进一步制备。给出了对未染色的纤毛虫（梨形四膜虫）、人血和不同花粉粒等不同细胞群体进行高速测量的示例。
