Visser T J, Klootwijk W, Docter R, Hennemann G
Neuroendocrinology. 1976;21(3):204-13. doi: 10.1159/000122606.
The inactivation of the hypothalamic hormone by rat liver and kidney homogenates was studied, using specific radioimmunoassays for the measurement of thyrotropin releasing hormone, pGlu-His-Pro-NH2 (TRH), and for an analogous peptide, pGlu-His-Pro-OH(TRH-OH), which has been proposed as a major metabolite of TRH [NAIR et al., 1971]. The inactivation of TRH and the free acid was found to be rapid. Heat lability and saturation kinetics suggest the involvement of enzymatic processes. In liver homogenate, TRH-OH production from TRH was observed. The accumulation of TRH-OH was substantial in experiments employing near-saturation concentrations of TRH. The liver and kidney are ascribed as major sites for breakdown of TRH in vivo.
利用特异性放射免疫分析法测定促甲状腺激素释放激素pGlu-His-Pro-NH2（TRH）以及一种类似肽pGlu-His-Pro-OH（TRH-OH）（有人提出它是TRH的主要代谢产物[奈尔等人，1971年]），对大鼠肝脏和肾脏匀浆使下丘脑激素失活的情况进行了研究。发现TRH和游离酸的失活很快。热不稳定性和饱和动力学表明有酶促过程参与。在肝脏匀浆中，观察到由TRH产生TRH-OH。在采用接近饱和浓度TRH的实验中，TRH-OH的积累量很大。肝脏和肾脏被认为是体内TRH分解的主要部位。
