Delfini C, Garbuglia A R, Alfani E, Di Caro A, Sette P, Benedetto A
Laboratory of Cell Biology, Istituto Superiore di Sanità, Rome, Italy.
J Med Virol. 1993 Oct;41(2):114-9. doi: 10.1002/jmv.1890410206.
Several reports show that the prevalence of HBV (hepatitis B virus) carriers in HIV (human immunodeficiency virus) infected populations is significantly higher than in HIV seronegative individuals, independent of the risk group for HIV, that is, homosexuals or drug abusers. In this context, evaluation of the simultaneous presence of HBV and HIV in PBMCs (peripheral blood mononuclear cells) is of particular interest for at least 2 reasons: 1) the possible reciprocal influence of the 2 viruses when they infect the same cell; 2) the possibility that HIV-induced hematological disorders could indirectly influence the settling of HBV in blood cell populations. We report data on the frequency of PCR positivity for HBV DNA in PBMCs from 62 HIV infected patients, rigorously selected by risk group, that is, intravenous use of heroin for at least 3 years and syringe promiscuity. Sixty-seven HIV negative individuals who never used any drug formed the control group. The analysis of the cases positive for HBV DNA in PBMCs showed that: 1) the overall prevalence of PCR positivity found in HIV infected patients was significantly lower than that registered in the control group; 2) PCR positivity among the subjects who were HBsAg negative and anti-HBV positive was extremely low in the HIV infected patients (3.7%) but quite frequent in the HIV negative subjects (37.0%). The results support the hypothesis that, unlike the HIV negative individuals, our HIV infected patients exhibited HBV DNA in PBMCS almost exclusively when they presented with active HBV replication.
几份报告显示，在感染人类免疫缺陷病毒（HIV）的人群中，乙肝病毒（HBV）携带者的患病率显著高于HIV血清阴性个体，这与HIV的风险群体无关，即与同性恋者或药物滥用者无关。在此背景下，评估外周血单个核细胞（PBMCs）中同时存在HBV和HIV的情况尤为重要，原因至少有两点：1）两种病毒感染同一细胞时可能存在的相互影响；2）HIV诱导的血液系统疾病可能间接影响HBV在血细胞群体中的定植。我们报告了62例HIV感染患者PBMCs中HBV DNA的PCR阳性频率数据，这些患者是根据风险群体严格挑选的，即静脉注射海洛因至少3年且共用注射器。67名从未使用过任何药物的HIV阴性个体组成了对照组。对PBMCs中HBV DNA阳性病例的分析表明：1）在HIV感染患者中发现的PCR阳性总体患病率显著低于对照组；2）在HBsAg阴性且抗HBV阳性的受试者中，PCR阳性在HIV感染患者中极低（3.7%），但在HIV阴性受试者中相当常见（37.0%）。结果支持这样的假设，即与HIV阴性个体不同，我们的HIV感染患者几乎仅在出现活跃的HBV复制时，PBMCs中才会出现HBV DNA。
