Rogers S D, Pearcy M J, Hay S J, Haynes D R, Bramley A, Howie D W
Department of Orthopaedic Surgery and Trauma, Royal Adelaide Hospital, Australia.
J Orthop Res. 1993 Nov;11(6):856-64. doi: 10.1002/jor.1100110612.
The wear of joint prostheses generates wear particles that produce an inflammatory response in the surrounding tissues and may contribute to bone resorption resulting in prosthetic loosening. Although the effects of particles produced from prosthetic materials have been studied extensively in vitro and in vivo, little attention has been paid to the standardisation of methods for the generation and characterization of these particles. This paper describes a reproducible method for generation of metal particles by the abrasive shaking of joint replacement components. Particular attention was given to the production of metal particles that closely resembled particles found around solid and loose human prostheses. To achieve this, particle size, size distribution, chemical composition, and shape were characterized. Particles that were 0.5-3.0 microns in diameter were isolated by differential sedimentation, and the distribution of particle sizes was determined with use of a Coulter Multisizer. Chemical composition was measured by atomic absorption spectrophotometry, and transmission electron microscopy was used to characterize particle shape. The techniques were shown to be reproducible, since there was little variation between batches over a lengthy time period. These or similar methods of particle production and characterization should be an essential part of future in vitro and in vivo studies of wear particles.
关节假体的磨损会产生磨损颗粒，这些颗粒会在周围组织中引发炎症反应，并可能导致骨吸收，进而造成假体松动。尽管已在体外和体内广泛研究了假体材料产生的颗粒的影响，但对于这些颗粒的产生和表征方法的标准化却鲜有关注。本文描述了一种通过对关节置换部件进行研磨振荡来产生金属颗粒的可重复方法。特别关注了产生与人类固定和松动假体周围发现的颗粒非常相似的金属颗粒。为实现这一目标，对颗粒的大小、大小分布、化学成分和形状进行了表征。通过差速沉降分离出直径为0.5 - 3.0微米的颗粒，并使用库尔特多通道粒度分析仪测定颗粒大小分布。通过原子吸收分光光度法测量化学成分，并使用透射电子显微镜表征颗粒形状。这些技术被证明具有可重复性，因为在很长一段时间内批次之间几乎没有差异。这些或类似的颗粒产生和表征方法应成为未来磨损颗粒体外和体内研究的重要组成部分。
