Kolbanovskaia E Iu, Morozov N Iu, Gavriushov S A, Il'in V A, Beintmena J, Vlodaver A, Karpeĭskiĭ M Ia
Mol Biol (Mosk). 1993 Nov-Dec;27(6):1315-34.
A detailed analysis of noncovalent interactions in the RNase A molecule was carried out. For this purpose, contact maps based on structural data were constructed. Three hydrophobic nuclei and five microclusters were identified, and their quantitative parameters were calculated. The contacts between amino acid residues of the active center and the hydrophobic nuclei were established. The distribution of the charged amino acid residues on the of RNase A surface was shown. The data obtained was discussed in the context of the ribonuclease A family. The substitutions seen from the alignment of amino acid sequences in the family might have influence on the spatial structure of different parts of RNase A and their mutual orientation and, consequently on the biological activity of family members.
对核糖核酸酶A分子中的非共价相互作用进行了详细分析。为此，基于结构数据构建了接触图谱。识别出三个疏水核和五个微簇，并计算了它们的定量参数。确定了活性中心的氨基酸残基与疏水核之间的接触。展示了核糖核酸酶A表面带电荷氨基酸残基的分布。在核糖核酸酶A家族的背景下对所得数据进行了讨论。从该家族氨基酸序列比对中观察到的取代可能会影响核糖核酸酶A不同部分的空间结构及其相互取向，进而影响家族成员的生物活性。
