Szot P, Dorsa D M
Geriatric Research, Education and Clinical Center (GRECC), Veterans Affairs Medical Center, Seattle, Wash. 98108.
Neuroendocrinology. 1993 Oct;58(4):381-7. doi: 10.1159/000126567.
Previous studies from this and other laboratories have shown that levels of vasopressin (VP) mRNA are reduced in both hypothalamic magnocellular and extrahypothalamic nuclei of the homozygous Brattleboro rat (HOM) when compared to the normal Long-Evans (LE) and heterozygous Brattleboro rats (HET). Since extrahypothalamic VP gene expression is dependent on testosterone (T), we measured plasma T in HOM, HET and LE rats. The plasma T level of the intact HOM rat was not significantly different from intact LE or HET rats. Manipulation of circulating gonadal steroids by castration and T replacement was found to regulate the expression of VP mRNA in the bed nucleus of the stria terminalis and medial amygdala of LE and HET rats, but does not appear to modify the absence of VP mRNA in neurons in these nuclei in the HOM rat.
本实验室及其他实验室之前的研究表明，与正常的长 Evans 大鼠（LE）和杂合型 Brattleboro 大鼠（HET）相比，纯合型 Brattleboro 大鼠（HOM）下丘脑大细胞和下丘脑外核中的血管加压素（VP）mRNA 水平降低。由于下丘脑外 VP 基因表达依赖于睾酮（T），我们测定了 HOM、HET 和 LE 大鼠的血浆 T 水平。完整的 HOM 大鼠的血浆 T 水平与完整的 LE 或 HET 大鼠无显著差异。通过去势和 T 替代来操纵循环性腺类固醇，发现其可调节 LE 和 HET 大鼠终纹床核和内侧杏仁核中 VP mRNA 的表达，但似乎并不能改变 HOM 大鼠这些核中神经元缺乏 VP mRNA 的情况。
